Rabbit NFIA Polyclonal Antibody | anti-NFIA antibody

NFIA (KIAA1439, Nuclear Factor 1 A-type, CCAAT-box-binding Transcription Factor, Nuclear Factor I/A, TGGCA-binding Protein) APC

Cat. Num. AAA6386953

• Gene Names: NFIA; CTF; NF1-A; NFI-A; NFI-L; NF-I/A
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NFIA; Polyclonal Antibody; NFIA (KIAA1439; Nuclear Factor 1 A-type; CCAAT-box-binding Transcription Factor; Nuclear Factor I/A; TGGCA-binding Protein) APC; anti-NFIA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NFIA. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-NFIA antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human NFIA, aa1-498 (NP_005586.1).
• Immunogen Sequence: MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDRPPDPFSTL
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NFIA rabbit polyclonal antibody. Western Blot analysis of NFIA expression in rat brain.)

Western Blot (WB)

(NFIA rabbit polyclonal antibody. Western Blot analysis of NFIA expression in mouse intestine.)

Western Blot (WB)

(Western Blot analysis of NFIA expression in transfected 293T cell line by NFIA polyclonal antibody. Lane 1: NFIA transfected lysate (54.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-NFIA antibody

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.

Product Categories/Family for anti-NFIA antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 30840980
• NCBI GeneID: 4774
• NCBI Accession #: NP_005586.1
• NCBI GenBank Nucleotide #: NM_005595.1
• UniProt Accession #: Q12857; Q8TA97; Q9H3X9; Q9P2A9; B4DRJ3; B4DS53; F5H0R0; F8W8W3
• Molecular Weight: 60,894 Da
• NCBI Official Full Name: nuclear factor 1 A-type isoform 2
• NCBI Official Synonym Full Names: nuclear factor I/A
• NCBI Official Symbol: NFIA
• NCBI Official Synonym Symbols: CTF; NF1-A; NFI-A; NFI-L; NF-I/A
• NCBI Protein Information: nuclear factor 1 A-type; TGGCA-binding protein; CCAAT-box-binding transcription factor
• UniProt Protein Name: Nuclear factor 1 A-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIA
• UniProt Synonym Gene Names: KIAA1439; NF1-A; Nuclear factor 1/A; CTF; NF-I/A; NFI-A
• UniProt Entry Name: NFIA_HUMAN

NCBI Description

This gene encodes a member of the NF1 (nuclear factor 1) family of transcription factors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

NFI-A: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Belongs to the CTF/NF-I family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1p31.3-p31.2

Cellular Component: nucleoplasm; nucleus; cell junction

Molecular Function: transcription factor binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; DNA replication; response to organic cyclic substance