Rabbit NFATC1 Polyclonal Antibody | anti-NFATC1 antibody

Anti-NFATC1 Antibody

Cat. Num. AAA419688

• Gene Names: NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: NFATC1; Polyclonal Antibody; Anti-NFATC1 Antibody; anti-NFATC1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of extracts of various cell lines, using NFATC1 antibody. Immunofluorescence analysis of U2OS cell using NFATC1 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 353

• Applicable Applications for anti-NFATC1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human NFATC1
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunofluorescence (IF)

Related Product Information for anti-NFATC1 antibody

The NFAT (nuclear factor of activated T cells) family of proteins consists of NFAT1 (NFATc2 or NFATp), NFAT2 (NFATc1 or NFATc), NFAT3 (NFATc4), and NFAT4 (NFATc3 or NFATx). All members of this family are transcription factors with a Rel homology domain and regulate gene transcription in concert with AP-1 (Jun/Fos) to orchestrate an effective immune response (1, 2). NFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation programs in these cells (3). In resting cells, NFAT proteins are heavily phosphorylated and localized in the cytoplasm. Increased intracellular calcium concentrations activate the calcium/calmodulin-dependent serine phosphatase calcineurin, which dephosphorylates NFAT proteins, resulting in their subsequent translocation to the nucleus (2). Termination of NFAT signaling occurs upon declining calcium concentrations and phosphorylation of NFAT by kinases such as GSK-3 or CK1 (3, 4). Cyclosporin A and FK506 are immunosuppressive drugs that inhibit calcineurin and thus retain NFAT proteins in the cytoplasm (5).

NCBI and Uniprot Product Information

• NCBI GI #: 27502388
• NCBI GeneID: 4772
• NCBI Accession #: NP_765976.1
• NCBI GenBank Nucleotide #: NM_172388.2
• UniProt Accession #: O95644; Q12865; Q15793; Q2M1S3; B5B2M4; B5B2M5; B5B2M6; B5B2M7; B5B2M8; B5B2M9; B5B2N1
• Molecular Weight: 38,626 Da
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 1 isoform D
• NCBI Official Synonym Full Names: nuclear factor of activated T-cells 1
• NCBI Official Symbol: NFATC1
• NCBI Official Synonym Symbols: NFAT2; NFATc; NF-ATC; NF-ATc1.2
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 1
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 1
• Protein Family: Nuclear factor of activated T-cells
• UniProt Gene Name: NFATC1
• UniProt Synonym Gene Names: NFAT2; NFATC; NF-ATc1; NFATc1; NF-ATc; NFATc

NCBI Description

The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. The product of this gene is an inducible nuclear component. It functions as a major molecular target for the immunosuppressive drugs such as cyclosporin A. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Different isoforms of this protein may regulate inducible expression of different cytokine genes. [provided by RefSeq, Jul 2013]

Uniprot Description

NFAT2: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Also control gene expression in embryonic cardiac cells. Could regulate not only the activation and proliferation but also the differentiation and programmed death of lymphoid and nonlymphoid cells. Six splice variant isoforms have been identified. Expressed in thymus, peripheral leukocytes as T-cells and spleen. Isoforms A are preferentially expressed in effector T-cells (thymus and peripheral leukocytes) whereas isoforms B and isoforms C are preferentially expressed in naive T-cells (spleen). Isoforms B are expressed in naive T-cells after first antigen exposure and isoforms A are expressed in effector T-cells after second antigen exposure.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 18q23

Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: FK506 binding; mitogen-activated protein kinase p38 binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: calcineurin-NFAT signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; transcription from RNA polymerase II promoter; Wnt receptor signaling pathway, calcium modulating pathway