Rabbit anti-Human, Mouse NFAT3 Polyclonal Antibody | anti-NFAT3 antibody

NFAT3 (Phospho-Ser676) Antibody

Cat. Num. AAA9502503

• Gene Names: NFATC4; NFAT3; NF-AT3; NF-ATC4
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: NFAT3; Polyclonal Antibody; NFAT3 (Phospho-Ser676) Antibody; NF-AT3; NF-ATc4; NFAC4; NFATC4; NFC4; Nuclear factor of activated T-cells; cytoplasmic 4; T cell transcription factor NFAT3; Transcription complex subunit NF-ATc4; anti-NFAT3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: NFAT3 (Phospho-Ser676) Antibody detects endogenous levels of NFAT3 only when phosphorylated at Ser676.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 82

• Applicable Applications for anti-NFAT3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; ELISA: 1:1000
• Target Modification: Phospho
• Modification Sites: H:S676; M:S676
• Immunogen: The antiserum was produced against synthesized peptide derived from human NFAT3 around the phosphorylation site of Ser676.
• Immunogen Range: 642-691
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40uM 30', using NFAT3 (Phospho-Ser676) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 209862845
• NCBI GeneID: 4776
• NCBI Accession #: NP_001129494.1
• NCBI GenBank Nucleotide #: NM_001136022.2
• UniProt Accession #: Q14934; B4DDG5; B4DY55; B5B2U7; B5B2U8; B5B2U9; B5B2V0; B5B2V1; B5B2V2; B5B2V3; B5B2V4; B5B2V5
• Molecular Weight: 95 kDa
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 4 isoform 1
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 4
• NCBI Official Symbol: NFATC4
• NCBI Official Synonym Symbols: NFAT3; NF-AT3; NF-ATC4
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 4
• UniProt Gene Name: NFATC4
• UniProt Synonym Gene Names: NFAT3; NF-ATc4; NFATc4; NF-AT3

NCBI Description

This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

Uniprot Description

Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 and IL-4. Transcriptionally repressed by estrogen receptors; this inhibition is further enhanced by estrogen. Increases the transcriptional activity of PPARG and has a direct role in adipocyte differentiation. May play an important role in myotube differentiation. May play a critical role in cardiac development and hypertrophy. May play a role in deafferentation-induced apoptosis of sensory neurons.