Rabbit anti-Human NFAT1 Polyclonal Antibody | anti-NFAT1 antibody

NFAT1 Polyclonal Antibody

Cat. Num. AAA3013118

• Gene Names: NFATC2; NFAT1; NFATP
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: ProA affinity purified
• Synonyms: NFAT1; Polyclonal Antibody; NFAT1 Polyclonal Antibody; NF-ATc2; NFATc2; NF-ATp; NFATC2; AI607462; cytoplasmic 2; KIAA0611; NF ATc2; NF ATp; NFAC2; NFAT 1; NFAT pre existing subunit; NFAT pre-existing subunit; NFAT transcription complex; preexisting component; NFAT1-D; NFATp; Nuclear factor of activated T cells cytoplasmic 2; Nuclear factor of activated T cells cytoplasmic calcineurin dependent 2; Nuclear factor of activated T cells pre-existing component; Nuclear factor of activated T cells; Nuclear factor of activated T-cells; Preexisting nuclear factor of activated T cells 2; T cell transcription factor NFAT 1; T-cell transcription factor NFAT1; anti-NFAT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NFAT1 polyclonal antibody detects endogenous levels of NFAT1 protein.
• Purity/Purification: ProA affinity purified
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 925

• Applicable Applications for anti-NFAT1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: WB: 1:500; ICC: 1:50-1:200; IHC: 1:50-1:200; FC/FACS: 1:50-1:100
• Immunogen: recombinant protein
• Preparation and Storage: Store at 4 degree C after thawing.; Aliquot store at -20 degree C or -80 degree C.; Avoid repeated freeze/thaw cycles.

Immunocytochemistry (ICC)

(ICC staining NFAT1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining NFAT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-NFAT1 antibody

The NFAT (nuclear factor of activated T cells) family of transcription factors regulates cytokine expression in T cells. Members of the family include NFATc1 (NFATc), NFATc2 (NFATp), NFATn, NFATc3 (NFAT4, NFATx) and NFATc4 (NFAT3). Recognition of antigen by the T cell receptor (TCR) eventually activates the calcium-dependent protein phosphatase calcineurin. Once activated, calcineurin stimulates the translocation of NFATc1 (cytoplasmic) from the cytoplasm to the nucleus where it associates with NFATn (nuclear). Like NFATc1, NFATc2 resides in the cytoplasm and translocates to the nucleus subsequent to activation of calcineurin. Once in the nucleus, NFATc2 synergizes with AP-1 transcription factors to initiate transcription of cytokine genes. NFATc3 and NFATc4 share 65% sequence identity with other members of the NFAT family. They are similar to NFATc2 in that they also synergize with the AP-1 family of proteins.

NCBI and Uniprot Product Information

• NCBI GI #: 27886541
• NCBI GeneID: 4773
• NCBI Accession #: NP_775114.1
• NCBI GenBank Nucleotide #: NP_775114.1
• UniProt Accession #: Q13469
• Molecular Weight: 135kDa
• NCBI Official Full Name: nuclear factor of activated T-cells, cytoplasmic 2 isoform C
• NCBI Official Synonym Full Names: nuclear factor of activated T cells 2
• NCBI Official Symbol: NFATC2
• NCBI Official Synonym Symbols: NFAT1; NFATP
• NCBI Protein Information: nuclear factor of activated T-cells, cytoplasmic 2
• UniProt Protein Name: Nuclear factor of activated T-cells, cytoplasmic 2
• UniProt Gene Name: NFATC2
• UniProt Synonym Gene Names: NFAT1; NFATP; NF-ATc2; NFATc2; NF-ATp
• UniProt Entry Name: NFAC2_HUMAN

NCBI Description

This gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants encoding different isoforms have been characterized. [provided by RefSeq, Apr 2012]

Uniprot Description

NFAT1: a transcription factor that plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha and GM-CSF. It is present in a phosphorylated the cytosol of unstimulated T cells. After T-cell activation, it is dephosphorylated by calcineurin and translocates to the nucleus as a part of a transcription complex. Two splice variant isoforms have been identified.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q13.2

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; nucleus; cytosol; ribonucleoprotein complex; actin cytoskeleton

Molecular Function: protein binding; DNA binding; transcription factor activity

Biological Process: response to drug; B cell receptor signaling pathway; cell migration; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of B cell proliferation; cytokine production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus