Western Blot (WB)
(Western blot analysis of lysate from MCF-7cells, using NF-M antibody.)
Rabbit anti-Human NF-M Polyclonal Antibody | anti-NF-M antibody
NF-M Antibody
Gene Names
NEFM; NFM; NEF3; NF-M
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
NF-M; Polyclonal Antibody; NF-M Antibody; 160 kDa neurofilament protein; NEF3; NEFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament triplet M protein; anti-NF-M antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
NF-M antibody detects endogenous levels of NF-M.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
540
Applicable Applications for anti-NF-M antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:10000
ELISA: 1:10000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human NF-M.
Immunogen Range
542-591
Preparation and Storage
Stable at -20 degree C for at least 1 year.
Western Blot (WB)
(Western blot analysis of lysate from MCF-7cells, using NF-M antibody.)
Western Blot (WB)
(Western blot analysis of lysate from MCF-7cells, using NF-M antibody.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102 kDa
NCBI Official Full Name
neurofilament medium polypeptide isoform 1
NCBI Official Synonym Full Names
neurofilament medium
NCBI Official Symbol
NEFM
NCBI Official Synonym Symbols
NFM; NEF3; NF-M
NCBI Protein Information
neurofilament medium polypeptide
UniProt Protein Name
Neurofilament medium polypeptide
UniProt Gene Name
NEFM
UniProt Synonym Gene Names
NEF3; NFM; NF-M
NCBI Description
Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and functionally maintain neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the medium neurofilament protein. This protein is commonly used as a biomarker of neuronal damage. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]
Uniprot Description
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Research Articles on NF-M
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Product Notes
The NF-M nefm (Catalog #AAA9508954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NF-M Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NF-M can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:10000. Researchers should empirically determine the suitability of the NF-M nefm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF-M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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