Western Blot (WB)
(Western blot analysis of NF-kappaB p65 (pS529) expression in MCF7 UV-treated (A); Raw264.7 TNFa-treated (B); rat liver (C); rat kidney (D) whole cell lysates.)
Rabbit NF kappaB p65 Polyclonal Antibody
NF kappaB p65 Antibody
Applications
Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
Immunogen Affinity Chromatography
Synonyms
NF kappaB p65; Polyclonal Antibody; NF kappaB p65 Antibody; Purified Polyclonal NF kappaB p65 Antibody; Polyclonal NF kappaB p65 antibody; Anti-NF kappaB p65 antibody; NFKB3 antibody; Transcription factor p65 antibody; Nuclear factor NF-kappa-B p65 subunit antibody; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody; NfKBp65 antibody; anti-NF kappaB p65 antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of NF-kappaB p65 protein
Purity/Purification
Immunogen Affinity Chromatography
Form/Format
Supplied in liquid form in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3 with 30% Glycerol and 0.01% Sodium Azide
Applicable Applications for anti-NF kappaB p65 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:1000
IHC: 1:100-1:200
IP: 1:10-1:100
IHC: 1:100-1:200
IP: 1:10-1:100
Immunogen
NF kappaB p65 antibody was raised in Rabbit using a KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human NF-kappaB p65 (pS529) as the immunogen
Cross-Reactivity
Mouse, Rat, Monkey
Preparation and Storage
Store at 4 degree C for short term storage. For long term, aliquot and store at -20 degree C. Avoid repeat freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of NF-kappaB p65 (pS529) expression in MCF7 UV-treated (A); Raw264.7 TNFa-treated (B); rat liver (C); rat kidney (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of NF-kappaB p65 (pS529) expression in MCF7 UV-treated (A); Raw264.7 TNFa-treated (B); rat liver (C); rat kidney (D) whole cell lysates.)
Related Product Information for anti-NF kappaB p65 antibody
NF kappaB p65 is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.
Product Categories/Family for anti-NF kappaB p65 antibody
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Product Notes
The NF kappaB p65 (Catalog #AAA5316244) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's NF kappaB p65 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:100-1:200 IP: 1:10-1:100. Researchers should empirically determine the suitability of the NF kappaB p65 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NF kappaB p65, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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