Rabbit anti-Rat Neurite Outgrowth Inhibitor Protein C Polyclonal Antibody
Anti-Neurite Outgrowth Inhibitor Protein C (NogoC)
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Serum
Synonyms
Neurite Outgrowth Inhibitor Protein C; Polyclonal Antibody; Anti-Neurite Outgrowth Inhibitor Protein C (NogoC); NogoC; anti-Neurite Outgrowth Inhibitor Protein C antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The rat peptide sequence is 100% conserved in human Nogo-C/foocen-S. Most of this region is unique to Nogo-C and not conserved in Nogo-A/B isoforms. Antibody crossreactivity of NogoC31 in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity
Purity/Purification
Serum
Form/Format
Supplied as a liquid, neat serum, 40% glycerol.
Applicable Applications for anti-Neurite Outgrowth Inhibitor Protein C antibody
ELISA; Western Blot
Application Notes
Recommended Dilutions: Western Blotting(1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. Nogo-C has a MW of ~25kD (1).
ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well.
Immunogen
An 11-aa peptide within the N-terminus (putative extracellular domain) of rat/human Nogo-C (1) conjugated to KLH
Source Information
Rat
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Temperature: Blue Ice
Shipping Temperature: Blue Ice
Related Product Information for anti-Neurite Outgrowth Inhibitor Protein C antibody
Introduction: Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrowafter injury. However, the CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses. Most recently an inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized. It may help block the regeneration of the CNS. Nogo is the 4th member of reticulon (Rtn) family. There are three alternative isoforms of Nogo, designated Nogo-A (full length human protein 1192 aa; calculated mol wt 135kD; rat 1163 aa), an intermediate form Nogo-B (373 aa; ~37 K, lacks 186-1004 aa within the extracellular domain), and a shorter form Nogo-C (199 aa; ~25 K, similar to rat vp20 and foocen-s; lacks 186-1004 aa but which has a smaller, alternative N-terminal domain). Nogo-A has a putative extracellular domain of 1024aa, 2-3 TM domains, and a short C-terminus of 43aa. Nogo-A is localized to the CNS-myelin, and is highly expressed in oligodendorcytes but not by Schwann cells. Nogo-B and Nogo-C have been found in several non-neuronal tissues (skeletal muscle, kidney, skin, lung, and spleen), and it may be the 35-kD protein recognized by IN-1 antibody.
Product Categories/Family for anti-Neurite Outgrowth Inhibitor Protein C antibody
Similar Products
Product Notes
The Neurite Outgrowth Inhibitor Protein C (Catalog #AAA623478) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Neurite Outgrowth Inhibitor Protein C (NogoC) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Neurite Outgrowth Inhibitor Protein C can be used in a range of immunoassay formats including, but not limited to, ELISA; Western Blot. Recommended Dilutions: Western Blotting(1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. Nogo-C has a MW of ~25kD (1). ELISA:1:10,000-1:100,000 using 50-100ng of control peptide/well. Researchers should empirically determine the suitability of the Neurite Outgrowth Inhibitor Protein C for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neurite Outgrowth Inhibitor Protein C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
