Western Blot (WB)
(Western blot analysis Neuralized-1 using MCF7 whole cell lysates)
Rabbit anti-Human Neuralized-1 Polyclonal Antibody | anti-NEURL1 antibody
Neuralized-1 Antibody
Gene Names
NEURL1; neu; NEUR1; NEURL; RNF67; neu-1; bA416N2.1
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Neuralized-1; Polyclonal Antibody; Neuralized-1 Antibody; h neu; h neuralized 1; Neu 1; Neu; Neuralized homolog; Neuralized like protein 1; Neuralized like protein 1A; NEURL1; RING finger protein 67; RNF67; anti-NEURL1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Neuralized-1 antibody detects endogenous levels of total Neuralized-1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
574
Applicable Applications for anti-NEURL1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human Neuralized-1
Subcellular Location
Cell Membrane; Peripheral Membrane Protein. Cytoplasm; Perinuclear Region.
Tissue Specificity
Expressed in brain, testis, pituitary gland, pancreas and bone marrow. Also poorly expressed in an malignant astrocytomas and several neuroectodermal tumor cell lines. Weakly expressed in medulloblastoma (MB) compared with normal cerebellar tissues.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis Neuralized-1 using MCF7 whole cell lysates)
Western Blot (WB)
(Western blot analysis Neuralized-1 using MCF7 whole cell lysates)
Immunofluorescene (IF)
(MBS9602169 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9602169 staining MCF7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-NEURL1 antibody
Function: Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway.
Subunit Structure: Interacts with CPEB3 (via N-terminal domain); the interaction increases CPEB3 ubiquitination. Interacts with DLL1.
Post-translational Modifications: Myristoylation is a determinant of membrane targeting.
Subunit Structure: Interacts with CPEB3 (via N-terminal domain); the interaction increases CPEB3 ubiquitination. Interacts with DLL1.
Post-translational Modifications: Myristoylation is a determinant of membrane targeting.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 62 kDa
Predicted: 62 kDa
Predicted: 62 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase NEURL1
NCBI Official Synonym Full Names
neuralized E3 ubiquitin protein ligase 1
NCBI Official Symbol
NEURL1
NCBI Official Synonym Symbols
neu; NEUR1; NEURL; RNF67; neu-1; bA416N2.1
NCBI Protein Information
E3 ubiquitin-protein ligase NEURL1
UniProt Protein Name
E3 ubiquitin-protein ligase NEURL1
Protein Family
UniProt Gene Name
NEURL1
UniProt Synonym Gene Names
NEURL; NEURL1A; RNF67; h-neu; h-neuralized 1
Uniprot Description
Plays a role in hippocampal-dependent synaptic plasticity, learning and memory. Involved in the formation of spines and functional synaptic contacts by modulating the translational activity of the cytoplasmic polyadenylation element-binding protein CPEB3. Promotes ubiquitination of CPEB3, and hence induces CPEB3-dependent mRNA translation activation of glutamate receptor GRIA1 and GRIA2. Can function as an E3 ubiquitin-protein ligase to activate monoubiquitination of JAG1 (in vitro), thereby regulating the Notch pathway. Acts as a tumor suppressor; inhibits malignant cell transformation of medulloblastoma (MB) cells by inhibiting the Notch signaling pathway.
Research Articles on NEURL1
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Product Notes
The NEURL1 neurl1 (Catalog #AAA9602169) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neuralized-1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neuralized-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NEURL1 neurl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neuralized-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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