Rabbit anti-Human, Mouse NEU4 Polyclonal Antibody | anti-NEU4 antibody

NEU4, CT (NEU4, Sialidase-4, N-acetyl-alpha-neuraminidase 4) (MaxLight 490)

Cat. Num. AAA6324779

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NEU4; Polyclonal Antibody; CT (NEU4; Sialidase-4; N-acetyl-alpha-neuraminidase 4) (MaxLight 490); N-acetyl-alpha-neuraminidase 4; anti-NEU4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NEU4. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.

• Applicable Applications for anti-NEU4 antibody: Western Blot (WB), Immunohistochemistry (IHC), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 423-452 in the C-terminal region of human NEU4, UniProt Accession #Q8WWR8.
• Conjugate: MaxLight490
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS6009069. This demonstrates that MBS6009069 detected the NEU4 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human hepatocarcinoma using MBS6009069 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6009069 for immunohistochemistry.)

Related Product Information for anti-NEU4 antibody

The protein encoded by this gene belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.

Product Categories/Family for anti-NEU4 antibody

Antibodies; Enzymes; Glycosidase

References

1. Bigi, A., et al. Glycobiology 20(2):148-157(2010). 2. Xin, X., et al. Genome Res. 19(7):1262-1269(2009). 3. Miyagi, T., et al. Proteomics 8(16):3303-3311(2008). 4. Stamatos, N.M., et al. FEBS J. 272(10):2545-2556(2005). 5. Hillier, L.W., et al. Nature 434(7034):724-731 (2005).

NCBI and Uniprot Product Information

• NCBI GI #: 262527265
• NCBI GeneID: 129807
• NCBI Accession #: NP_001161072.1; NP_542779.2; NP_001161074.1; NP_001161071.1; NP_001161073.1
• NCBI GenBank Nucleotide #: NM_001167600.2
• UniProt Accession #: Q8WWR8; Q96D64; A8K056; J3KNJ5
• Molecular Weight: 53,066 Da
• NCBI Official Full Name: sialidase-4 isoform 3
• NCBI Official Synonym Full Names: neuraminidase 4
• NCBI Official Symbol: NEU4
• NCBI Protein Information: sialidase-4
• UniProt Protein Name: Sialidase-4
• Protein Family: Sialidase
• UniProt Gene Name: NEU4
• UniProt Entry Name: NEUR4_HUMAN

NCBI Description

The protein encoded by this gene belongs to a family of glycohydrolytic enzymes, which remove terminal sialic acid residues from various sialo derivatives, such as glycoproteins, glycolipids, oligosaccharides, and gangliosides. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

NEU4: May function in lysosomal catabolism of sialylated glycoconjugates. Has sialidase activity towards synthetic substrates, such as 2'-(4-methylumbelliferyl)-alpha-D-N- acetylneuraminic acid (4-MU-NANA or 4MU-NeuAc). Has a broad substrate specificity being active on glycoproteins, oligosaccharides and sialylated glycolipids. Belongs to the glycosyl hydrolase 33 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - sphingolipid; EC 3.2.1.18; Hydrolase; Glycan Metabolism - other glycan degradation

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: lysosomal lumen; organelle inner membrane; mitochondrion; lysosome

Molecular Function: protein binding; exo-alpha-sialidase activity

Biological Process: oligosaccharide catabolic process; cellular protein metabolic process; sphingolipid metabolic process; dolichol-linked oligosaccharide biosynthetic process; ganglioside catabolic process; glycosphingolipid metabolic process; protein amino acid N-linked glycosylation via asparagine; glycoprotein catabolic process; post-translational protein modification