Rabbit anti-Human NEU2 Polyclonal Antibody | anti-NEU2 antibody

NEU2, NT (NEU2, Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2) (Azide free) (HRP)

Cat. Num. AAA6324766

• Gene Names: NEU2; SIAL2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: NEU2; Polyclonal Antibody; NT (NEU2; Sialidase-2; Cytosolic sialidase; N-acetyl-alpha-neuraminidase 2) (Azide free) (HRP); N-acetyl-alpha-neuraminidase 2; anti-NEU2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NEU2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NEU2 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 23-50 in the N-terminal region of human NEU2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in A549 cell line lysates (35ug/lane) usingMBS6003454. This demonstrates that MBS6003454 detected the NEU2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue using MBS6003454 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6003454 for immunohistochemistry.)

Testing Data

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Testing Data

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Product Categories/Family for anti-NEU2 antibody

Antibodies; Enzymes; Glycosidase

References

1. Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009). 2. Li, C.Y., et al. Cell Res. 17(4):357-362(2007). 3. Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005). 4. Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004). 5. Tringali, C., et al. J. Biol. Chem. 279(5):3169-3179(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 222352170
• NCBI GeneID: 4759
• NCBI Accession #: NP_005374.2
• NCBI GenBank Nucleotide #: NM_005383.2
• UniProt Accession #: Q9Y3R4; Q3KNW4; Q6NTB4
• Molecular Weight: 42,254 Da
• NCBI Official Full Name: sialidase-2
• NCBI Official Synonym Full Names: neuraminidase 2
• NCBI Official Symbol: NEU2
• NCBI Official Synonym Symbols: SIAL2
• NCBI Protein Information: sialidase-2
• UniProt Protein Name: Sialidase-2
• Protein Family: Sialidase
• UniProt Gene Name: NEU2

NCBI Description

This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the removal of sialic acid (N-acetylneuraminic acid) moities from glycoproteins, oligosaccharides and gangliosides.