Goat NET1 Polyclonal Antibody | anti-NET1 antibody

Goat Polyclonal to Human NET1

Cat. Num. AAA241645

• Gene Names: NET1; NET1A; ARHGEF8
• Reactivity: Gorilla, Human; Predicted Reactivity: Orangutan, Gibbon, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NET1; Polyclonal Antibody; Goat Polyclonal to Human NET1; Anti-NET1 Antibody (C-Terminus) IHC-plus; ARHGEF8; NET1A; Proto-oncogene p65 Net1; Human NET1; anti-NET1 antibody

• Host: Goat
• Reactivity: Gorilla, Human; Predicted Reactivity: Orangutan, Gibbon, Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human NET1. This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 542

• Applicable Applications for anti-NET1 antibody: Immunohistochemistry (IHC - Paraffin), ELISA (EIA)
• Application Notes: ELISA (1:32000), IHC-P (2.5 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-DKALSGGKRKETLV from the C-terminus of human NET1 (NP_001040625.1; NP_005854.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Orangutan, Gibbon, Monkey (93%); Marmoset, Mouse, Hamster (86%).
• Immunogen Type: Synthetic peptide
• Immunogen: NET1 antibody was raised against synthetic peptide C-DKALSGGKRKETLV from the C-terminus of human NET1 (NP_001040625.1; NP_005854.2). Percent identity by BLAST analysis: Human, Gorilla (100%); Orangutan, Gibbon, Monkey (93%); Marmoset, Mouse, Hamster (86%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-NET1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 19923327
• NCBI GeneID: 10276
• NCBI Accession #: NP_005854.2
• NCBI GenBank Nucleotide #: NM_005863.4
• UniProt Accession #: Q7Z628; Q12773; Q96D82; Q99903; Q9UEN6
• Molecular Weight: 61,885 Da
• NCBI Official Full Name: neuroepithelial cell-transforming gene 1 protein isoform 2
• NCBI Official Synonym Full Names: neuroepithelial cell transforming 1
• NCBI Official Symbol: NET1
• NCBI Official Synonym Symbols: NET1A; ARHGEF8
• NCBI Protein Information: neuroepithelial cell-transforming gene 1 protein; p65 Net1 proto-oncogene protein; small GTP-binding protein regulator; neuroepithelioma transforming gene 1; Rho guanine nucleotide exchange factor (GEF) 8; guanine nucleotide regulatory protein (oncogene)
• UniProt Protein Name: Neuroepithelial cell-transforming gene 1 protein
• Protein Family: Nucleolar protein
• UniProt Gene Name: NET1
• UniProt Synonym Gene Names: ARHGEF8
• UniProt Entry Name: ARHG8_HUMAN

NCBI Description

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

NET1: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; GEFs; GEFs, Rac/Rho

Chromosomal Location of Human Ortholog: 10p15

Cellular Component: plasma membrane; cytosol; nucleus

Molecular Function: Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; GTP-Rho binding

Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; small GTPase mediated signal transduction; myoblast migration; regulation of cell growth; signal transduction