Rabbit anti-Human Nerve Growth Factor, beta Polyclonal Antibody | anti-NGF antibody

Nerve Growth Factor, beta (NGFb)

Cat. Num. AAA618444

• Gene Names: NGF; NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428
• Reactivity: Human
• Applications: ELISA, Western Blot, Neutralization Assay
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Nerve Growth Factor; beta; Polyclonal Antibody; beta (NGFb); Anti -Nerve Growth Factor; anti-NGF antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human NGFb.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.2. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

• Applicable Applications for anti-NGF antibody: ELISA (EL/EIA), Western Blot (WB), Neutralization
• Application Notes: Suitable for use in Direct ELISA, Western Blot and Neutralization.; Dilution: Direct ELISA: 0.5ug/ml using 100ul/well. Detects 0.2-0.4ng/well of recombinant human NGFb.; Western Blot: 0.1-0.2ug/ml. The detection limit for recombinant human NGFb is 1.5-3ng/lane, under either reducing or non-reducing conditions.; Neutralization: 0.05-0.08ug/ml yields one-half maximal inhibition (ND50) of the biological activity of 1ng/ml human NGFb.; Optimal dilution to be determined by the researcher.
• Immunogen: Highly pure recombinant human NGFb.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NGF antibody

Nerve growth factor-b (b-NGF) is a potent neurotrophic factor which supports the growth and survivability of nerve and/or glial cells. The active form of human b-NGF is a dimer, formed by two identical 119 amino acid subunits, which is held together by strong hydrophobic interactions.

Product Categories/Family for anti-NGF antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 70995319
• NCBI GeneID: 4803
• NCBI Accession #: NP_002497.2
• NCBI GenBank Nucleotide #: NM_002506.2
• UniProt Accession #: P01138; Q6FHA0; Q96P60; Q9P2Q8; Q9UKL8; A1A4E5
• Molecular Weight: 26,959 Da
• NCBI Official Full Name: beta-nerve growth factor
• NCBI Official Synonym Full Names: nerve growth factor (beta polypeptide)
• NCBI Official Symbol: NGF
• NCBI Official Synonym Symbols: NGFB; HSAN5; Beta-NGF; MGC161426; MGC161428
• NCBI Protein Information: beta-nerve growth factor; OTTHUMP00000013653; nerve growth factor, beta subunit
• UniProt Protein Name: Beta-nerve growth factor
• Protein Family: Nerve growth factor
• UniProt Gene Name: NGF
• UniProt Synonym Gene Names: NGFB
• UniProt Entry Name: NGF_HUMAN

NCBI Description

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq]

Uniprot Description

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p13.1

Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome

Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity

Biological Process: response to nicotine; circadian rhythm; response to peptide hormone stimulus; activation of MAPKK activity; nerve growth factor receptor signaling pathway; adult locomotory behavior; positive regulation of apoptosis; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of axonogenesis; regulation of caspase activity; sensory perception of pain; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; inflammatory response; response to electrical stimulus; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; positive regulation of nerve growth factor receptor signaling pathway; memory; regulation of release of sequestered calcium ion into cytosol; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; phospholipase C activation; response to mechanical stimulus; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V