Rabbit anti-Rat Nephrin Related Protein 1 Polyclonal Antibody | anti-Nphs1 antibody

Nephrin Related Protein 1 (NEPH1)

Cat. Num. AAA610847

• Gene Names: Nphs1; nephrin; NephrinB
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Nephrin Related Protein 1; Polyclonal Antibody; Nephrin Related Protein 1 (NEPH1); Anti -Nephrin Related Protein 1 (NEPH1); anti-Nphs1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat NEPH1. Species Sequence Homology: human, mouse - 100%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, before the addition of glycerol to 40%.

• Applicable Applications for anti-Nphs1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA, Western Blot.; Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See N2028-62A.; Western Blot (ECL): 1-10ug/ml.
• Immunogen: A synthetic 16aa peptide from rat NEPH1. Epitope: ~C-terminus, Cytoplasmic domain.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Nphs1 antibody

Nephrin is encoded by NPHS1 gene (chromosome 19q13.1), the core protein of the interpodocytes slit diaphragm of kidney glomerulus, these podocytes are highly differentiated with characteristic interdigitating foot processes covering the outer glomerular basement membrane, the space between these foot processes is spanned by a tight junction to provide the large surface area for filtration. The mutated NPHS1 gene in congenital nephrotic syndrome of Finnish type, a human disease that leads to massive proteinuria in utero and nephrosis at birth. Nephrin is a transmembrane protein of immunoglobulin super family with 8 Ig like domains, identified in human (1241aa), rat (1252aa), and mouse (1256aa).

Product Categories/Family for anti-Nphs1 antibody

Antibodies; Abs to Nephrin

NCBI and Uniprot Product Information

• NCBI GI #: 120586997
• NCBI GeneID: 54631
• NCBI Accession #: NP_062332.2
• NCBI GenBank Nucleotide #: NM_019459.2
• UniProt Accession #: Q9QZS7; Q811S5; Q925S5; Q9ESC6; Q9ET59; Q9JIX1; D2KXA7
• Molecular Weight: 136,336 Da
• NCBI Official Full Name: nephrin
• NCBI Official Synonym Full Names: nephrosis 1, nephrin
• NCBI Official Symbol: Nphs1
• NCBI Official Synonym Symbols: nephrin; NephrinB
• NCBI Protein Information: nephrin; nephrin 1; nephrosis 1 homolog, nephrin; renal glomerulus-specific cell adhesion receptor
• UniProt Protein Name: Nephrin
• Protein Family: Nephrin
• UniProt Gene Name: Nphs1
• UniProt Synonym Gene Names: Nphn
• UniProt Entry Name: NPHN_MOUSE

Uniprot Description

NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Cellular Component: protein complex; cell projection; membrane; integral to plasma membrane; integral to membrane; plasma membrane; intracellular; lipid raft

Molecular Function: protein domain specific binding; protein binding; spectrin binding; myosin binding; alpha-actinin binding

Biological Process: skeletal muscle development; muscle development; positive regulation of actin filament polymerization; multicellular organismal development; MAPKKK cascade; JNK cascade; cell adhesion; myoblast fusion