Rabbit anti-Human Nephrin (NPHN) Polyclonal Antibody | anti-NPHN antibody

PE-Linked Polyclonal Antibody to Nephrin (NPHN)

Cat. Num. AAA2117914

• Gene Names: NPHS1; CNF; NPHN; nephrin
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Nephrin (NPHN); Polyclonal Antibody; PE-Linked Polyclonal Antibody to Nephrin (NPHN); NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor; anti-NPHN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against NPHN. It has been selected for its ability to recognize NPHN in immunohistochemical staining and western blotting.
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid; 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: >=100ug/ml (varies by lot)
• Sequence: Pro27~Val234

• Applicable Applications for anti-NPHN antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
• Immunogen Sequence: Pro27~Val234
• Cross Reactivity: Human, Mouse, Rat
• Conjugation: PE
• Preparation and Storage: Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.; Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for anti-NPHN antibody

Tumor Immunity; Kidney Biomarker

NCBI and Uniprot Product Information

• NCBI GI #: 10441644
• NCBI GeneID: 4868
• UniProt Accession #: O60500; A6NDH2; C3RX61
• Molecular Weight: 134,742 Da
• NCBI Official Full Name: nephrin
• NCBI Official Synonym Full Names: nephrosis 1, congenital, Finnish type (nephrin)
• NCBI Official Symbol: NPHS1
• NCBI Official Synonym Symbols: CNF; NPHN; nephrin
• NCBI Protein Information: nephrin; renal glomerulus-specific cell adhesion receptor
• UniProt Protein Name: Nephrin
• UniProt Gene Name: NPHS1
• UniProt Synonym Gene Names: NPHN
• UniProt Entry Name: NPHN_HUMAN

NCBI Description

This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.[provided by RefSeq, Oct 2009]

Uniprot Description

NPHS1: Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion. Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL/NEPH1 and KIRREL2; the interaction with KIRREL is dependent on KIRREL glycosylation. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1. Interacts with NPHS2. Specifically expressed in podocytes of kidney glomeruli. Belongs to the immunoglobulin superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Immunoglobulin superfamily; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cell projection; protein complex; integral to plasma membrane; plasma membrane; lipid raft

Molecular Function: protein domain specific binding; protein binding; myosin binding; spectrin binding; alpha-actinin binding

Biological Process: skeletal muscle development; positive regulation of actin filament polymerization; glomerular basement membrane development; JNK cascade; excretion; cell adhesion; myoblast fusion

Disease: Nephrotic Syndrome, Type 1