Western Blot (WB)
(Western Blot from this lot. A431 lysate was resolved by electrophoresis, transferred to membrane and probed with MBS612660 (1:500). Proteins were visualized using a donkey anti-rabbit IgG (HRP) and a chemiluminescence detection system. Lane 1: pUSE untransfected control; Lane 2: Transfected cells.)
Rabbit Neomycin Phosphotransferase II Polyclonal Antibody | anti-NPTII antibody
Neomycin Phosphotransferase II (NPT II) (MaxLight 490)
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography
Synonyms
Neomycin Phosphotransferase II; Polyclonal Antibody; Neomycin Phosphotransferase II (NPT II) (MaxLight 490); anti-NPTII antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes bacterial NPT II, Mr ~30kD.
Purity/Purification
Purified by Protein A Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight490.
Sequence Length
264
Applicable Applications for anti-NPTII antibody
FLISA, Western Blot (WB)
Application Notes
WB: Detects NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene.
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Full length Neomycin Phosphotransferase II (NPT II)
Conjugate
MaxLight490
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot from this lot. A431 lysate was resolved by electrophoresis, transferred to membrane and probed with MBS612660 (1:500). Proteins were visualized using a donkey anti-rabbit IgG (HRP) and a chemiluminescence detection system. Lane 1: pUSE untransfected control; Lane 2: Transfected cells.)
Western Blot (WB)
(Western Blot from this lot. A431 lysate was resolved by electrophoresis, transferred to membrane and probed with MBS612660 (1:500). Proteins were visualized using a donkey anti-rabbit IgG (HRP) and a chemiluminescence detection system. Lane 1: pUSE untransfected control; Lane 2: Transfected cells.)
Product Categories/Family for anti-NPTII antibody
References
1. Qian, Y., et al., Biochem. J. 415: 123-134 (2008). General References: 1. Fuchs, R.L., et al., Biotechnology 11: 1537-1542 (1993). 2. Fuchs, M. and Gonsalves, D., Biotechnology 13: 1466-1473 (1995). 3. Horn, D. and Cross, G.A.M., Cell 83: 555-561 (1995). 4. Hu, H., et al., Neuron 16: 735-743 (1996).
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NPTII (Catalog #AAA6489712) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Neomycin Phosphotransferase II can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB). WB: Detects NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene. Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NPTII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neomycin Phosphotransferase II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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