Rabbit anti-Human NEK10 Polyclonal Antibody | anti-NEK10 antibody

NEK10 Antibody (Center)

Cat. Num. AAA9215706

• Reactivity: Human
• Applications: Western Blot
• Synonyms: NEK10; Polyclonal Antibody; NEK10 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Serine/threonine-protein kinase Nek10; Never in mitosis A-related kinase 10; NimA-related protein kinase 10; anti-NEK10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This NEK10 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 450-483 amino acids from the Central region of human NEK10.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 474

• Applicable Applications for anti-NEK10 antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-NEK10 Antibody (Center) at 1:2000 dilutionLane 1: A431 whole cell lysatesLane 2: Hela whole cell lysatesLane 3: HepG2 whole cell lysatesLane 4: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 133 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-NEK10 antibody

Signal Transduction

References

Ota T.,et al.Nat. Genet. 36:40-45(2004). Li H.,et al.Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases. Muzny D.M.,et al.Nature 440:1194-1198(2006). Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases. Bechtel S.,et al.BMC Genomics 8:399-399(2007).

NCBI and Uniprot Product Information

• NCBI GI #: 72534776
• NCBI GeneID: 152110
• NCBI Accession #: NP_001026911.1
• NCBI GenBank Nucleotide #: NM_001031741.3
• UniProt Accession #: Q6ZWH5; Q45VJ4; Q6ZR11; Q7Z671; Q86XB1; Q96MB3; A8MWG1; B9ZVR0
• Molecular Weight: 133259
• NCBI Official Full Name: serine/threonine-protein kinase Nek10 isoform 1
• NCBI Official Synonym Full Names: NIMA-related kinase 10
• NCBI Official Symbol: NEK10
• NCBI Protein Information: serine/threonine-protein kinase Nek10
• UniProt Protein Name: Serine/threonine-protein kinase Nek10
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: NEK10
• UniProt Synonym Gene Names: NimA-related protein kinase 10
• UniProt Entry Name: NEK10_HUMAN

NCBI Description

NEK10: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Other group; NEK family

Chromosomal Location of Human Ortholog: 3p24.1

Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of MAP kinase activity; positive regulation of protein amino acid autophosphorylation; protein amino acid phosphorylation

Uniprot Description

NEK10: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Other; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Other group; NEK family

Chromosomal Location of Human Ortholog: 3p24.1

Cellular Component: centrosome; cytoplasm; nucleus

Molecular Function: ATP binding; metal ion binding; protein kinase activity; protein serine/threonine kinase activity

Biological Process: chromosome segregation; microtubule-based process; positive regulation of MAP kinase activity; positive regulation of protein amino acid autophosphorylation; protein amino acid phosphorylation