Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Goat NEIL1/NEH1 Polyclonal Antibody | anti-NEIL1 antibody
Goat anti-NEIL1/NEH1 Antibody
Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1.
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
((5ug/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Uniprot Description
NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.
Protein type: Lyase; EC 4.2.99.18; Hydrolase; DNA repair, damage
Chromosomal Location of Human Ortholog: 15q24.2
Cellular Component: chromosome; cytoplasm; microtubule organizing center; nucleoplasm; nucleus
Molecular Function: damaged DNA binding; DNA N-glycosylase activity; DNA-(apurinic or apyrimidinic site) lyase activity; hydrolase activity, acting on glycosyl bonds; protein C-terminus binding; zinc ion binding
Biological Process: base-excision repair; depyrimidination; negative regulation of nuclease activity; nucleotide-excision repair; response to oxidative stress
Research Articles on NEIL1
Similar Products
Product Notes
The NEIL1 neil1 (Catalog #AAA420005) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NEIL1/NEH1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NEIL1/NEH1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 16000. Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10ug/ml. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. Researchers should empirically determine the suitability of the NEIL1 neil1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DIPSLEPEGT SAS. It is sometimes possible for the material contained within the vial of "NEIL1/NEH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
