Western Blot (WB)
(Western Blot analysis using MBS6000456 in MCF-7 cell line lysates (35ug/lane). This demonstrates MBS6000456 detected the NEDD9 protein (arrow).)
Rabbit anti-Human NEDD9 Polyclonal Antibody
NEDD9, ID (NEDD9, CASL, CASS2, Enhancer of filamentation 1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12, p10
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
NEDD9; Polyclonal Antibody; ID (NEDD9; CASL; CASS2; Enhancer of filamentation 1; CRK-associated substrate-related protein; Cas scaffolding protein family member 2; Neural precursor cell expressed developmentally down-regulated protein 9; Renal carcinoma antigen NY-REN-12; p10; Anti -NEDD9; anti-NEDD9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-NEDD9 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Immunogen
NEDD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 334-363 amino acids from the Central region of human NEDD9.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis using MBS6000456 in MCF-7 cell line lysates (35ug/lane). This demonstrates MBS6000456 detected the NEDD9 protein (arrow).)
Western Blot (WB)
(Western Blot analysis using MBS6000456 in MCF-7 cell line lysates (35ug/lane). This demonstrates MBS6000456 detected the NEDD9 protein (arrow).)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using MBS6000456 in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemistry analysis using MBS6000456 in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.)
Related Product Information for anti-NEDD9 antibody
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B-and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
Product Categories/Family for anti-NEDD9 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The NEDD9 (Catalog #AAA6000456) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEDD9, ID (NEDD9, CASL, CASS2, Enhancer of filamentation 1, CRK-associated substrate-related protein, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, Renal carcinoma antigen NY-REN-12, p10 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD9 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot, Immunohistochemistry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Immunohistochemistry: 1:10-50. Researchers should empirically determine the suitability of the NEDD9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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