Western Blot (WB)
(Western blot analysis of extracts of mouse brain tissue, using NEDD8 antibody.)
Rabbit NEDD8 Polyclonal Antibody | anti-NEDD8 antibody
NEDD8 (Neural Precursor Cell-expressed Developmentally Down-regulated Gene 8, FLJ43224, MGC104393, MGC125896, MGC125897, Nedd-8) (HRP)
Gene Names
NEDD8; NEDD-8
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by affinity chromatography.
Synonyms
NEDD8; Polyclonal Antibody; NEDD8 (Neural Precursor Cell-expressed Developmentally Down-regulated Gene 8; FLJ43224; MGC104393; MGC125896; MGC125897; Nedd-8) (HRP); anti-NEDD8 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes NEDD8. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-NEDD8 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Applications are based on unconjugated antibody.
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human NEDD8.
Conjugate
HRP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts of mouse brain tissue, using NEDD8 antibody.)
Western Blot (WB)
(Western blot analysis of extracts of mouse brain tissue, using NEDD8 antibody.)
Product Categories/Family for anti-NEDD8 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,072 Da
NCBI Official Full Name
NEDD8
NCBI Official Synonym Full Names
neural precursor cell expressed, developmentally down-regulated 8
NCBI Official Symbol
NEDD8
NCBI Official Synonym Symbols
NEDD-8
NCBI Protein Information
NEDD8; neddylin; ubiquitin-like protein Nedd8
UniProt Protein Name
NEDD8
Protein Family
UniProt Gene Name
NEDD8
UniProt Synonym Gene Names
NEDD-8
UniProt Entry Name
NEDD8_HUMAN
Uniprot Description
NEDD8: a member of the ubiquitin family of proteins. Approximately 60% identical to ubiquitin protein. Activated by an E1-like complex, consisting of App-b1 and Uba3 and then linked to the E2-like enzyme, Ubc12. The major target protein modified by nedd8 is cullin-4a. Down-regulated during the development of brain.
Protein type: Ubiquitin-like modifier
Chromosomal Location of Human Ortholog: 14q12
Cellular Component: cytosol; nucleus
Molecular Function: protein binding; ubiquitin protein ligase binding
Biological Process: regulation of transcription from RNA polymerase II promoter; ubiquitin-dependent protein catabolic process; anatomical structure morphogenesis; protein localization; protein neddylation; transforming growth factor beta receptor signaling pathway; protein modification process; proteolysis; response to organic cyclic substance
Research Articles on NEDD8
Similar Products
Product Notes
The NEDD8 nedd8 (Catalog #AAA6428722) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NEDD8 (Neural Precursor Cell-expressed Developmentally Down-regulated Gene 8, FLJ43224, MGC104393, MGC125896, MGC125897, Nedd-8) (HRP) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NEDD8 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NEDD8 nedd8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NEDD8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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