Goat NEBL/nebulette Polyclonal Antibody | anti-NEBL antibody

Goat anti-NEBL/nebulette Antibody

Cat. Num. AAA421779

• Gene Names: NEBL; LASP2; LNEBL
• Reactivity: Expected from sequence similarity: Human, Mouse, Dog
• Applications: ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: NEBL/nebulette; Polyclonal Antibody; Goat anti-NEBL/nebulette Antibody; NEBL; nebulette; FLJ53769; LNEBL; MGC119746; MGC119747; bA56H7.1; LIM-nebulette; actin-binding Z-disc protein; OTTHUMP00000019268; actin-binding Z-disk protein; NEBL antibody; nebulette antibody; FLJ53769 antibody; LNEBL antibody; MGC119746 antibody; MGC119747 antibody; bA56H7.1 antibody; LIM-nebulette antibody; actin-binding Z-disc protein antibody; OTTHUMP00000019268 antibody; actin-binding Z-disk protein antibody; Nebulette; NEBL / nebulette; anti-NEBL antibody

• Host: Goat
• Reactivity: Expected from sequence similarity: Human, Mouse, Dog
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: ELQRLKRTQE
• Sequence Length: 270

• Applicable Applications for anti-NEBL antibody: Peptide ELISA (EIA)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 4000.; Western Blot: Preliminary experiments gave an approx 30kDa band in Human Brain (Frontal Cortex) lysates, but not in Human, Mouse and Rat Heart lysates after 1ug/ml antibody staining. This protein has a calculated MW of 31.2kDa according to NP_998734.1. The 30kDa band was successfully blocked by incubation with the immunizing peptide.
• Immunogen: Peptide with sequence C-ELQRLKRTQE, from the internal region of the protein sequence according to NP_998734.1; NP_001166955.1.
• Epitope: Internal region
• Note: This antibody is expected to recognize isoform 2 and isoform 3 (NP_998734.1; NP_001166955.1) only, but does not recognize isoform 1 (NP_006384.1).
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 47087157
• NCBI GeneID: 10529
• NCBI Accession #: NP_998734.1; NP_001166955.1
• NCBI GenBank Nucleotide #: NM_213569.2
• Molecular Weight: 31,196 Da
• NCBI Official Full Name: nebulette isoform 2
• NCBI Official Synonym Full Names: nebulette
• NCBI Official Symbol: NEBL
• NCBI Official Synonym Symbols: LASP2; LNEBL
• NCBI Protein Information: nebulette
• UniProt Protein Name: Nebulette
• Protein Family: Nebulette
• UniProt Gene Name: NEBL
• UniProt Synonym Gene Names: LNEBL
• UniProt Entry Name: NEBL_HUMAN

NCBI Description

This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010]

Uniprot Description

NEBL: Binds to actin and plays an important role in the assembly of the Z-disk. Isoform 2 might play a role in the assembly of focal adhesion. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding

Chromosomal Location of Human Ortholog: 10p12

Cellular Component: I band; stress fiber; Z disc

Molecular Function: actin filament binding; cytoskeletal protein binding; filamin binding; protein binding; structural constituent of muscle; tropomyosin binding