Western Blot (WB)
((0.1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NDUFS8 Polyclonal Antibody | anti-NDUFS8 antibody
Goat anti-NDUFS8 Antibody
Western Blot: Approx 25kDa band observed in Mouse and Rat Heart lysates (calculated MW of 24. 0kDa according to Mouse NP_659119.2 and Rat NP_001099792.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot (WB)
((0.1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Mouse Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a subunit of mitochondrial NADH:ubiquinone oxidoreductase, or Complex I, a multimeric enzyme of the respiratory chain responsible for NADH oxidation, ubiquinone reduction, and the ejection of protons from mitochondria. The encoded protein is involved in the binding of two of the six to eight iron-sulfur clusters of Complex I and, as such, is required in the electron transfer process. Mutations in this gene have been associated with Leigh syndrome. [provided by RefSeq, Mar 2010]
Uniprot Description
TYKY: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. May donate electrons to ubiquinone. Defects in NDUFS8 are a cause of Leigh syndrome (LS). LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Belongs to the complex I 23 kDa subunit family.
Protein type: EC 1.6.99.3; Oxidoreductase; EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial
Chromosomal Location of Human Ortholog: 11q13
Cellular Component: mitochondrial matrix; mitochondrial respiratory chain complex I; mitochondrion
Molecular Function: 4 iron, 4 sulfur cluster binding; metal ion binding; NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase activity
Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; response to oxidative stress
Disease: Leigh Syndrome
Research Articles on NDUFS8
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Product Notes
The NDUFS8 ndufs8 (Catalog #AAA422420) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NDUFS8 Antibody reacts with Tested: Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFS8 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 25kDa band observed in Mouse and Rat Heart lysates (calculated MW of 24. 0kDa according to Mouse NP_659119.2 and Rat NP_001099792.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the NDUFS8 ndufs8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EAEIAANIQA D. It is sometimes possible for the material contained within the vial of "NDUFS8, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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