Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat NDUFS3 Polyclonal Antibody | anti-NDUFS3 antibody
Goat anti-NDUFS3 Antibody
Western Blot: Approx 28kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 30.2kDa according to NP_004542.1). Recommended concentration: 0.3-1ug/ml.
Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.[provided by RefSeq, Apr 2009]
Uniprot Description
NDUFS3: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I 30 kDa subunit family.
Protein type: EC 1.6.5.3; Energy Metabolism - oxidative phosphorylation; Mitochondrial; Oxidoreductase; EC 1.6.99.3
Chromosomal Location of Human Ortholog: 11p11.11
Cellular Component: mitochondrial matrix; mitochondrial membrane; mitochondrial respiratory chain complex I; mitochondrion; myelin sheath; nucleus
Molecular Function: electron carrier activity; NADH dehydrogenase (ubiquinone) activity; NADH dehydrogenase activity; protein binding
Biological Process: mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly; negative regulation of cell growth; substantia nigra development
Disease: Leigh Syndrome; Mitochondrial Complex I Deficiency
Research Articles on NDUFS3
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Product Notes
The NDUFS3 ndufs3 (Catalog #AAA421828) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-NDUFS3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFS3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 4000. Western Blot: Approx 28kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 30.2kDa according to NP_004542.1). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the NDUFS3 ndufs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: DTRPTVRPRN DVAHK. It is sometimes possible for the material contained within the vial of "NDUFS3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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