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Western Blot (WB) (Western blot analysis of NDUFC2 expression in A375 (A), PC3 (B), H9C2 (C), AML12 (D) whole cell lysates.)

Rabbit NDUFC2 Polyclonal Antibody | anti-NDUFC2 antibody

Anti-NDUFC2 Antibody

Gene Names
NDUFC2; HLC-1; B14.5b; NADHDH2; CI-B14.5b
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
NDUFC2; Polyclonal Antibody; Anti-NDUFC2 Antibody; NADH dehydrogenase [ubiquinone] 1 subunit C2; Complex I-B14.5b; CI-B14.5b; Human lung cancer oncogene 1 protein; HLC-1; NADH-ubiquinone oxidoreductase subunit B14.5b; anti-NDUFC2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of NDUFC2 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-NDUFC2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NDUFC2. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of NDUFC2 expression in A375 (A), PC3 (B), H9C2 (C), AML12 (D) whole cell lysates.)

Western Blot (WB) (Western blot analysis of NDUFC2 expression in A375 (A), PC3 (B), H9C2 (C), AML12 (D) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NDUFC2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of NDUFC2 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Related Product Information for anti-NDUFC2 antibody
Rabbit polyclonal antibody to NDUFC2

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
14,188 Da
NCBI Official Full Name
NADH dehydrogenase
NCBI Official Synonym Full Names
NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa
NCBI Official Symbol
NDUFC2
NCBI Official Synonym Symbols
HLC-1; B14.5b; NADHDH2; CI-B14.5b
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 subunit C2; NADH dehydrogenase [ubiquinone] 1 subunit C2; complex I-B14.5b; complex I subunit B14.5b; human lung cancer oncogene 1 protein; NADH-ubiquinone oxidoreductase subunit B14.5b
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 subunit C2
Protein Family
UniProt Gene Name
NDUFC2
UniProt Synonym Gene Names
CI-B14.5b; HLC-1
UniProt Entry Name
NDUC2_HUMAN

Uniprot Description

NDUFC2: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFC2 subunit family.

Protein type: Membrane protein, integral; Mitochondrial; Energy Metabolism - oxidative phosphorylation

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: mitochondrial inner membrane; cytoplasm; integral to membrane; mitochondrial respiratory chain complex I

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone

Research Articles on NDUFC2

Similar Products

Product Notes

The NDUFC2 ndufc2 (Catalog #AAA8305535) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NDUFC2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:1000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NDUFC2 ndufc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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