Rabbit NDUFB7 Polyclonal Antibody | anti-NDUFB7 antibody

NDUFB7 Polyclonal Antibody

Cat. Num. AAA3006835

• Gene Names: NDUFB7; B18; CI-B18
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: NDUFB7; Polyclonal Antibody; NDUFB7 Polyclonal Antibody; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7; Cell adhesion protein SQM1; Complex I-B18; CI-B18; NADH-ubiquinone oxidoreductase B18 subunit; anti-NDUFB7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: NDUFB7 polyclonal antibody detects endogenous levels of NDUFB7 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 137

• Applicable Applications for anti-NDUFB7 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human NDUFB7.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of NDUFB7 polyclonal antibody at 1:500 dilutionLane1:Hela whole cell lysateLane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate)

Related Product Information for anti-NDUFB7 antibody

Complex 1 (also known as NADH dehydrogenase) of the electron transport chain (respiratory chain) is an enzymatic complex that catalyzes the transfer of electrons from NADH to ubiquinone. Free energy from the reaction is conserved in the transfer of protons into the intermembrane space to create an electrochemical proton gradient, a driving force for ATP synthesis. Complex 1 is a complicated, multi-protein, L-shaped complex composed of at least 45 different subunits and located in the mitochondrial inner membrane. NDUB7 (NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7), also known as NADH-ubiquinone oxioreductase B18 subunit, complex I-B18 (CI-B18) or cell adhesion protein SQM1, is a 137 amino acid accessory subunit of complex 1. Ubiquitously expressed, NDUFB7 localizes to the mitochondrial inner membrane on the matrix side. NDUFB7 contains a sevenfold repeat of positively-charged residues that may indicate a role in protein-protein interactions.

NCBI and Uniprot Product Information

• NCBI GI #: 10764847
• NCBI GeneID: 4713
• NCBI Accession #: NP_004137.2
• NCBI GenBank Nucleotide #: NP_004137.2
• UniProt Accession #: P17568
• Molecular Weight: ~ 16kDa
• NCBI Official Full Name: NADH dehydrogenase
• NCBI Official Synonym Full Names: NADH:ubiquinone oxidoreductase subunit B7
• NCBI Official Symbol: NDUFB7
• NCBI Official Synonym Symbols: B18; CI-B18
• NCBI Protein Information: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
• UniProt Protein Name: NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7
• Protein Family: NADH dehydrogenase
• UniProt Gene Name: NDUFB7
• UniProt Synonym Gene Names: CI-B18
• UniProt Entry Name: NDUB7_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the multisubunit NADH:ubiquinone oxidoreductase (complex I). Mammalian complex I is composed of 45 different subunits. It is located at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. [provided by RefSeq, Jul 2008]

Uniprot Description

NDUFB7: Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. Belongs to the complex I NDUFB7 subunit family.

Protein type: Energy Metabolism - oxidative phosphorylation; Oxidoreductase; EC 1.6.5.3; Mitochondrial; EC 1.6.99.3

Chromosomal Location of Human Ortholog: 19p13.12

Cellular Component: mitochondrial inner membrane; mitochondrial intermembrane space; mitochondrial respiratory chain complex I; mitochondrion

Molecular Function: NADH dehydrogenase (ubiquinone) activity

Biological Process: cellular metabolic process; mitochondrial electron transport, NADH to ubiquinone; mitochondrial respiratory chain complex I assembly