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Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: PC3 and hela cell, Primary antibody: 123632(NDUFB3 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Rabbit anti-Human NDUFB3 Polyclonal Antibody | anti-NDUFB3 antibody

NDUFB3 Rabbit pAb

Gene Names
NDUFB3; B12; CI-B12
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Affinity purified
Synonyms
NDUFB3; Polyclonal Antibody; NDUFB3 Rabbit pAb; B12; CI-B12; anti-NDUFB3 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Purity/Purification
Affinity purified
Form/Format
-20 degree C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Applicable Applications for anti-NDUFB3 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB: 1:500-1:2000
Immunogen
Full length fusion protein
Conjugation
Unconjugated
Molecular Function
This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency.
Modification
Unmodified
Cell Position
Membrane, Mitochondrion, Mitochondrion inner membrane
Preparation and Storage
Store at 4 degree C for short term. Store at -20 degree C for long term. Avoid freeze/thaw cycle.

Western Blot (WB)

(Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: PC3 and hela cell, Primary antibody: 123632(NDUFB3 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Western Blot (WB) (Gel: 12%SDS-PAGE, Lysate: 40 ug, Lane 1-2: PC3 and hela cell, Primary antibody: 123632(NDUFB3 Antibody) at dilution 1/350, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 123632(NDUFB3 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 123632(NDUFB3 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 123632(NDUFB3 Antibody) at dilution 1/25. (Original magnification: ×200))

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 123632(NDUFB3 Antibody) at dilution 1/25. (Original magnification: ×200))
Product Categories/Family for anti-NDUFB3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
11,402 Da
NCBI Official Full Name
Homo sapiens NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 3, 12kDa, mRNA
NCBI Official Synonym Full Names
NADH:ubiquinone oxidoreductase subunit B3
NCBI Official Symbol
NDUFB3
NCBI Official Synonym Symbols
B12; CI-B12
NCBI Protein Information
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
UniProt Protein Name
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3
Protein Family
UniProt Gene Name
NDUFB3
UniProt Synonym Gene Names
CI-B12
UniProt Entry Name
NDUB3_HUMAN

NCBI Description

This gene encodes an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) which is the first enzyme in the electron transport chain of mitochondria. This protein localizes to the inner membrane of the mitochondrion as a single-pass membrane protein. Mutations in this gene contribute to mitochondrial complex 1 deficiency. Alternative splicing results in multiple transcript variants encoding the same protein. Humans have multiple pseudogenes of this gene. [provided by RefSeq, Mar 2012]

Research Articles on NDUFB3

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Product Notes

The NDUFB3 ndufb3 (Catalog #AAA4754537) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NDUFB3 Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NDUFB3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NDUFB3 ndufb3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NDUFB3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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