Rabbit anti-Human NDUFAF1 Polyclonal Antibody | anti-NDUFAF1 antibody

NDUFAF1, CT (NDUFAF1, CIA30, Complex I intermediate-associated protein 30, mitochondrial, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1) (Azide free) (HRP)

Cat. Num. AAA6324390

• Gene Names: NDUFAF1; CGI65; CIA30; CGI-65
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NDUFAF1; Polyclonal Antibody; CT (NDUFAF1; CIA30; Complex I intermediate-associated protein 30; mitochondrial; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1) (Azide free) (HRP); NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 1; anti-NDUFAF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NDUFAF1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NDUFAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 250-280 amino acids from the C-terminal region of human NDUFAF1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NDUFAF1 Antibody (C-term) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the NDUFAF1 antibody detected the NDUFAF1 protein (arrow).)

Immunohistochemistry (IHC)

(NDUFAF1 antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NDUFAF1 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NDUFAF1 antibody

The NDUFAF1 gene encodes the human homolog of a Neurospora crassa Cia30 protein involved in the assembly of complex I.

Product Categories/Family for anti-NDUFAF1 antibody

Antibodies; Mitochondrial Proteins

References

Wheeler, H.E., et al. PLoS Genet. 5 (10), E1000685 (2009): Dunning, C.J., et al. EMBO J. 26(13):3227-3237(2007). Vogel, R.O., et al. Mol. Genet. Metab. 91(2):176-182(2007). Vogel, R.O., et al. Genes Dev. 21(5):615-624(2007). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 49574510
• NCBI GeneID: 51103
• NCBI Accession #: NP_057097.2
• NCBI GenBank Nucleotide #: NM_016013.3
• UniProt Accession #: Q9Y375; Q9BVZ5
• Molecular Weight: 37,764 Da
• NCBI Official Full Name: complex I intermediate-associated protein 30, mitochondrial
• NCBI Official Synonym Full Names: NADH dehydrogenase (ubiquinone) complex I, assembly factor 1
• NCBI Official Symbol: NDUFAF1
• NCBI Official Synonym Symbols: CGI65; CIA30; CGI-65
• NCBI Protein Information: complex I intermediate-associated protein 30, mitochondrial; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1; NADH-ubiquinone oxidoreductase 1 alpha subcomplex, assembly factor 1
• UniProt Protein Name: Complex I intermediate-associated protein 30, mitochondrial
• UniProt Gene Name: NDUFAF1
• UniProt Synonym Gene Names: CIA30
• UniProt Entry Name: CIA30_HUMAN

NCBI Description

This gene encodes a complex I assembly factor protein. Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19. [provided by RefSeq, Dec 2011]

Uniprot Description

NDUFAF1: Chaperone protein involved in the assembly of the mitochondrial NADH:ubiquinone oxidoreductase complex (complex I). Belongs to the CIA30 family.

Chromosomal Location of Human Ortholog: 15q11.2-q21.3

Cellular Component: cytoplasm; mitochondrial respiratory chain complex I

Molecular Function: protein binding; unfolded protein binding

Biological Process: mitochondrial electron transport, NADH to ubiquinone; protein complex assembly

Disease: Mitochondrial Complex I Deficiency