Rabbit anti-Human ND5 Polyclonal Antibody

ND5, CT (NADH-ubiquinone Oxidoreductase Chain 5, NADH Dehydrogenase Subunit 5, NADH5, Mitochondrially Encoded NADH Dehydrogenase 5, MTND5, MT-ND5)

Cat. Num. AAA6006325

• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ND5; Polyclonal Antibody; CT (NADH-ubiquinone Oxidoreductase Chain 5; NADH Dehydrogenase Subunit 5; NADH5; Mitochondrially Encoded NADH Dehydrogenase 5; MTND5; MT-ND5); Anti -ND5; anti-ND5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ND5.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide.

• Applicable Applications for anti-ND5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:1,000; Western Blot: 1:100-1:500
• Immunogen: Synthetic peptide selected from the C-terminal region of human ND5 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in CEM cell line lysates (35ug/lane) using MBS6006325. ND5 (arrow) was detected using MBS6006325.)

Testing Data

(All lanes: MBS6006325 (1:1000) Lane 1: CCRF-CEM whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 67kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human brain tissue reacted with MBS6006325, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS6006325 for immunohistochemistry.)

Related Product Information for anti-ND5 antibody

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

Product Categories/Family for anti-ND5 antibody

Antibodies; Abs to Enzymes; Dehydrogenase

NCBI and Uniprot Product Information

• NCBI GI #: 543172670
• NCBI Official Full Name: ND5 (mitochondrion)
• Protein Family: NADH-ubiquinone oxidoreductase

Uniprot Description

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.