Rabbit anti-Human NCX1 Polyclonal Antibody | anti-NCX1 antibody

NCX1, ID (Na+/Ca2+ Exchange Protein 1, CNC, DKFZp779F0871, MGC119581, Na+/Ca2+ Exchanger, NCX, Solute Carrier Family 8 Member 1, SLC8A1) (FITC)

Cat. Num. AAA6489509

• Gene Names: SLC8A1; NCX1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: NCX1; Polyclonal Antibody; ID (Na+/Ca2+ Exchange Protein 1; CNC; DKFZp779F0871; MGC119581; Na+/Ca2+ Exchanger; NCX; Solute Carrier Family 8 Member 1; SLC8A1) (FITC); anti-NCX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human SLC8A1.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-NCX1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-1:500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human SLC8A1 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of SLC8A1 Antibody (Center) in HL-60(lane 1), K562(lane 2) cell line lysates (35ug/lane). SLC8A1 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(SLC8A1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SLC8A1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SLC8A1 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-NCX1 antibody

Antibodies; Ion Channel

References

1. Palty, R., et.al., Proc. Natl. Acad. Sci. USA 107(1), 436-441 (2010). 2. Kepp, K., et.al., BMC Med. Genet. 11, 15 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 10863913
• NCBI GeneID: 6546
• NCBI Accession #: NP_066920.1
• NCBI GenBank Nucleotide #: NM_021097.2
• UniProt Accession #: P32418; O95849; Q4QQG6; Q587I6; Q59GN4; Q9UBL8; Q9UD55; Q9UDN1; Q9UDN2; Q9UKX6; A8K6N1; D6W595
• Molecular Weight: 107,938 Da
• NCBI Official Full Name: sodium/calcium exchanger 1 isoform A
• NCBI Official Synonym Full Names: solute carrier family 8 member 1
• NCBI Official Symbol: SLC8A1
• NCBI Official Synonym Symbols: NCX1
• NCBI Protein Information: sodium/calcium exchanger 1
• UniProt Protein Name: Sodium/calcium exchanger 1
• UniProt Gene Name: SLC8A1
• UniProt Synonym Gene Names: CNC; NCX1
• UniProt Entry Name: NAC1_HUMAN

NCBI Description

In cardiac myocytes, Ca(2+) concentrations alternate between high levels during contraction and low levels during relaxation. The increase in Ca(2+) concentration during contraction is primarily due to release of Ca(2+) from intracellular stores. However, some Ca(2+) also enters the cell through the sarcolemma (plasma membrane). During relaxation, Ca(2+) is sequestered within the intracellular stores. To prevent overloading of intracellular stores, the Ca(2+) that entered across the sarcolemma must be extruded from the cell. The Na(+)-Ca(2+) exchanger is the primary mechanism by which the Ca(2+) is extruded from the cell during relaxation. In the heart, the exchanger may play a key role in digitalis action. The exchanger is the dominant mechanism in returning the cardiac myocyte to its resting state following excitation.[supplied by OMIM, Apr 2004]

Uniprot Description

NCX1: Rapidly transports Ca(2+) during excitation-contraction coupling. Ca(2+) is extruded from the cell during relaxation so as to prevent overloading of intracellular stores. Belongs to the sodium/potassium/calcium exchanger family. SLC8 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transporter; Motility/polarity/chemotaxis; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: integral to plasma membrane; basolateral plasma membrane; T-tubule; plasma membrane; Z disc; sarcolemma

Molecular Function: calmodulin binding; metal ion binding; cytoskeletal protein binding; ankyrin binding; calcium:sodium antiporter activity

Biological Process: heart morphogenesis; cellular sodium ion homeostasis; embryonic placenta development; regulation of heart rate; vascular smooth muscle contraction; post-embryonic development; calcium ion homeostasis; cardiac muscle cell development; muscle contraction; reduction of cytosolic calcium ion concentration; embryonic heart tube development; ion transport; blood coagulation; muscle fiber development; transmembrane transport; regulation of the force of heart contraction; cardiac muscle contraction