Immunohistochemistry (IHC)
(Anti-NCS-1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Rabbit NCS1 / NCS-1 Polyclonal Antibody | anti-NCS1 antibody
Rabbit Polyclonal to Mouse NCS1 / NCS-1
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
Immunohistochemistry (IHC)
(Anti-NCS-1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC)
(Anti-NCS-1 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunofluorescence (IF)
(Anti-FREQUENIN Antibody - Immunofluorescence Microscopy. Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Gross et al., (2000) J. Neurosci. 20:1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numer-ous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.)
Immunofluorescence (IF)
(Anti-FREQUENIN Antibody - Immunofluorescence Microscopy. Hippocampal neurons were prepared from 17 d old NMRI mice and grown as described previously (G. Gross et al., (2000) J. Neurosci. 20:1869-1882). The figure shows the distribution of neuronal calcium sensor-1 (NCS-1) (red) and synaptobrevin (green) in hippocampal cell cultures after 19 d in vitro. Numer-ous synapses immunoreactive for synaptobrevin also show NCS-1 immunoreactivity. NCS-1 derived from invertebrate homologs has been referred to as frequenin.)
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. The protein encoded by this gene regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. The protein is associated with secretory granules and modulates synaptic transmission and synaptic plasticity. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
FREQ: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. Interacts with KCND2. Interacts in a calcium-independent manner with PI4KB. This binding competes with CALN2/CABP7 binding to PI4KB. Interacts with ARF1, ARF3, ARF5 and ARF6. Interacts in a calcium-dependent manner with PICK1 (via AH domain). Interacts with IL1RAPL1. Belongs to the recoverin family.
Protein type: Calcium-binding
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: postsynaptic membrane; intracellular membrane-bound organelle; axon; perinuclear region of cytoplasm; cytoplasm; postsynaptic density; dendrite; plasma membrane; cell junction; cytosol
Molecular Function: voltage-gated calcium channel activity; protein binding; magnesium ion binding; calcium ion binding; protein kinase binding
Biological Process: phosphoinositide-mediated signaling; positive regulation of exocytosis
Research Articles on NCS1
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Product Notes
The NCS1 ncs1 (Catalog #AAA241367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse NCS1 / NCS-1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCS1 / NCS-1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA). ELISA, IF, IHC-P (5 ug/ml), WB Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the NCS1 ncs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCS1 / NCS-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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