Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using NCOA6 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit NCOA6 Polyclonal Antibody | anti-NCOA6 antibody
NCOA6 Antibody
Gene Names
NCOA6; NRC; AIB3; ASC2; PRIP; TRBP; RAP250
Reactivity
Human, MousePredicted: Bovine, Horse, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NCOA6; Polyclonal Antibody; NCOA6 Antibody; anti-NCOA6 antibody
Host
Rabbit
Reactivity
Human, Mouse
Predicted: Bovine, Horse, Dog
Predicted: Bovine, Horse, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
NCOA6 Antibody detects endogenous levels of NCOA6.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1070
Applicable Applications for anti-NCOA6 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human NCOA6.
Tissue Specificity
Ubiquitous. Highly expressed in brain, prostate, testis and ovary; weakly expressed in lung, thymus and small intestine.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using NCOA6 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HEK-293 cells, using NCOA6 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NCOA6 antibody
Description: Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Coactivates expression in an agonist- and AF2-dependent manner. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ERs), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Probably functions as a general coactivator, rather than just a nuclear receptor coactivator. May also be involved in the coactivation of the NF-kappa-B pathway. May coactivate expression via a remodeling of chromatin and its interaction with histone acetyltransferase proteins.
Post Translational Modifications: Phosphorylated by PRKDC. Phosphorylation on Ser-884 leads to a strong decrease in binding to ESR1 and ESR2.
Subunit Structure: Monomer and homodimer. Interacts with RBM39 (By similarity). Interacts in vitro with the basal transcription factors GTF2A and TBP, suggesting an autonomous transactivation function. Interacts with NCOA1, CRSP3, RBM14, the histone acetyltransferases EP300 and CREBBP, and with the methyltransferases NCOA6IP and PRMT2/HRMT1L1. Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin. Interacts with ZNF335; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors.
Similarity: Contains two Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Only motif 1 is essential for the association with nuclear receptors, while adjacent Ser-884 displays selectivity for nuclear receptors.
Post Translational Modifications: Phosphorylated by PRKDC. Phosphorylation on Ser-884 leads to a strong decrease in binding to ESR1 and ESR2.
Subunit Structure: Monomer and homodimer. Interacts with RBM39 (By similarity). Interacts in vitro with the basal transcription factors GTF2A and TBP, suggesting an autonomous transactivation function. Interacts with NCOA1, CRSP3, RBM14, the histone acetyltransferases EP300 and CREBBP, and with the methyltransferases NCOA6IP and PRMT2/HRMT1L1. Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin. Interacts with ZNF335; may enhance ligand-dependent transcriptional activation by nuclear hormone receptors.
Similarity: Contains two Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. Only motif 1 is essential for the association with nuclear receptors, while adjacent Ser-884 displays selectivity for nuclear receptors.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 219 kDa
Predicted: 220 kDa
Predicted: 220 kDa
NCBI Official Full Name
nuclear receptor coactivator 6 isoform 2
NCBI Official Synonym Full Names
nuclear receptor coactivator 6
NCBI Official Symbol
NCOA6
NCBI Official Synonym Symbols
NRC; AIB3; ASC2; PRIP; TRBP; RAP250
NCBI Protein Information
nuclear receptor coactivator 6
UniProt Protein Name
Nuclear receptor coactivator 6
Protein Family
UniProt Gene Name
NCOA6
UniProt Synonym Gene Names
AIB3; KIAA0181; RAP250; TRBP; ASC-2; NRC RAP250; PPAR-interacting protein; PRIP
UniProt Entry Name
NCOA6_HUMAN
NCBI Description
The protein encoded by this gene is a transcriptional coactivator that can interact with nuclear hormone receptors to enhance their transcriptional activator functions. This protein has been shown to be involved in the hormone-dependent coactivation of several receptors, including prostanoid, retinoid, vitamin D3, thyroid hormone, and steroid receptors. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2011]
Research Articles on NCOA6
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Product Notes
The NCOA6 ncoa6 (Catalog #AAA9611926) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOA6 Antibody reacts with Human, Mouse Predicted: Bovine, Horse, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NCOA6 ncoa6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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