Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NCOA1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Rabbit NCOA1 Polyclonal Antibody | anti-NCOA1 antibody
NCOA1 Polyclonal Antibody
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NCOA1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NCOA1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using NCOA1 antibody. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U2OS cells using NCOA1 antibody. Blue: DAPI for nuclear staining.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Uniprot Description
SRC-1: a transcriptional coactivator for steroid and nuclear hormone receptors. A member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, and bHLH and PAS domains. Phosphorylated and activated via the mitogen-activated protein kinase pathway. Three alternatively spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; Acetyltransferase; Transcription, coactivator/corepressor; Oncoprotein; DNA-binding; EC 2.3.1.48
Chromosomal Location of Human Ortholog: 2p23
Cellular Component: nucleoplasm; neuron projection; nuclear chromatin; cytoplasm
Molecular Function: ligand-dependent nuclear receptor binding; protein dimerization activity; transcription coactivator activity; protein N-terminus binding; retinoid X receptor binding; protein binding; signal transducer activity; enzyme binding; histone acetyltransferase activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; protein complex binding; estrogen receptor binding; chromatin binding; progesterone receptor binding; nuclear hormone receptor binding
Biological Process: lactation; hypothalamus development; response to retinoic acid; male mating behavior; positive regulation of transcription from RNA polymerase II promoter by galactose; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; hippocampus development; male gonad development; positive regulation of female receptivity; cellular lipid metabolic process; response to estradiol stimulus; cellular response to hormone stimulus; ovulation cycle; androgen receptor signaling pathway; cerebellum development; positive regulation of transcription from RNA polymerase II promoter; cerebral cortex development; positive regulation of neuron differentiation; response to progesterone stimulus
Research Articles on NCOA1
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Product Notes
The NCOA1 ncoa1 (Catalog #AAA126769) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCOA1 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCOA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the NCOA1 ncoa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCOA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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