Western Blot (WB)
(Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Rabbit NCLN Polyclonal Antibody | anti-NCLN antibody
NCLN Antibody
Gene Names
NCLN; NET59
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
NCLN Antibody is affinity chromatography purified via peptide column.
Synonyms
NCLN; Polyclonal Antibody; NCLN Antibody; NET59; Nicalin; Nicastrin-like protein; nicalin; anti-NCLN antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
NCLN Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
563
Applicable Applications for anti-NCLN antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
NCLN antibody can be used for detection of NCLN by Western blot at 0.5 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 2.5 mug/mL.
Conjugate
Unconjugated
Immunogen
NCLN antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human NCLN.
Buffer
NCLN Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
NCLN antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Western Blot (WB)
(Western blot analysis of NCLN in mouse heart tissue lysate with NCLN antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.)
Immunohistochemistry (IHC)
(Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of NCLN in human heart tissue with NCLN antibody at 2.5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of NCLN in human heart tissue with NCLN antibody at 20 μg/mL.)
Related Product Information for anti-NCLN antibody
NCLN Antibody: Nicalin, along with its binding partner Nomo, is a novel signaling antagonist to Nodals, signaling factors of the TGF-β superfamily that play a key role in vertebrate development. Nicalin is distantly related to Nicastrin, a component of the Alzheimer's disease-associated gamma-secretase, and is thought to have a similar function. Another protein, TMEM147, has also been recently identified as associating with Nicalin and NOMO as part of the gamma-secretase-like complex.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
62,846 Da
NCBI Official Full Name
nicalin
NCBI Official Synonym Full Names
nicalin
NCBI Official Symbol
NCLN
NCBI Official Synonym Symbols
NET59
NCBI Protein Information
nicalin; nicastrin-like protein; nicalin homolog (zebrefish)
UniProt Protein Name
Nicalin
Protein Family
UniProt Gene Name
NCLN
UniProt Entry Name
NCLN_HUMAN
Uniprot Description
NCLN: May antagonize Nodal signaling and subsequent organization of axial structures during mesodermal patterning. Belongs to the nicastrin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: endoplasmic reticulum membrane; membrane; integral to membrane
Molecular Function: protein binding
Biological Process: regulation of signal transduction
Similar Products
Product Notes
The NCLN ncln (Catalog #AAA150400) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCLN Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NCLN can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). NCLN antibody can be used for detection of NCLN by Western blot at 0.5 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 2.5 mug/mL. Researchers should empirically determine the suitability of the NCLN ncln for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCLN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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