Rabbit anti-Human NCL Polyclonal Antibody | anti-NCL antibody

NCL, ID (E443) (NCL, Nucleolin, Protein C23) (Azide free) (HRP)

Cat. Num. AAA6324170

• Gene Names: NCL; C23
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NCL; Polyclonal Antibody; ID (E443) (NCL; Nucleolin; Protein C23) (Azide free) (HRP); Protein C23; anti-NCL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NCL antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NCL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 435-463 amino acids from the Central region of human NCL.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NCL Antibody (Center E443) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the NCL antibody detected the NCL protein (arrow).)

Western Blot (WB)

(Western blot analysis of NCL (arrow) using rabbit polyclonal NCL Antibody (Center E443). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NCL gene.)

Immunohistochemistry (IHC)

(NCL Antibody (Center E443) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NCL Antibody (Center E443) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Fluorescent confocal image of Hela cell stained with NCL Antibody (Center E443). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with NCL primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). NCL immunoreactivity is localized to nucleus significantly. )

Related Product Information for anti-NCL antibody

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.

Product Categories/Family for anti-NCL antibody

Antibodies; Nuclear Proteins

References

Ishimaru, D., et al. J. Biol. Chem. 285(35):27182-27191(2010). Tulchin, N., et al. Am. J. Pathol. 176(3):1203-1214(2010). Strang, B.L., et al. J. Virol. 84(4):1771-1784(2010). Bertrand, L., et al. J. Virol. 84(1):109-118(2010). Jerke, U., et al. PLoS ONE 4 (12), E8302 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 55956788
• NCBI GeneID: 4691
• NCBI Accession #: NP_005372.2
• NCBI GenBank Nucleotide #: NM_005381.2
• UniProt Accession #: P19338; Q53SK1; Q8NB06; Q9UCF0; Q9UDG1
• Molecular Weight: 76,614 Da
• NCBI Official Full Name: nucleolin
• NCBI Official Synonym Full Names: nucleolin
• NCBI Official Symbol: NCL
• NCBI Official Synonym Symbols: C23
• NCBI Protein Information: nucleolin
• UniProt Protein Name: Nucleolin
• Protein Family: Nucleolin
• UniProt Gene Name: NCL
• UniProt Entry Name: NUCL_HUMAN

NCBI Description

Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

Uniprot Description

NCL: the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and preribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-RNA transcription and ribosome assembly.

Protein type: Nuclear receptor co-regulator; DNA-binding; RNA-binding; Nucleolus; Helicase

Chromosomal Location of Human Ortholog: 2q37.1

Cellular Component: nucleoplasm; membrane; nucleolus; cell cortex; nucleus; ribonucleoprotein complex

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; RNA binding; nucleotide binding; telomeric DNA binding

Biological Process: angiogenesis