anti-Human NCKX1 Polyclonal Antibody | anti-NCKX1 antibody
Anti - NCKX1 (K+-dependent, Na Ca Exchanger 1)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified over the peptide-Sepharose column
Synonyms
NCKX1; Polyclonal Antibody; Anti - NCKX1 (K+-dependent; Na Ca Exchanger 1); NCKX1 (K+-dependent; anti-NCKX1 antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The 18 AA human control peptide is 57% conserved in bovine NCKX1. No significant sequence homology is detected with rat NCKX1 other NCX or NCKX2. The control peptide is available to confirm specificity of antibodies
Purity/Purification
Purified over the peptide-Sepharose column
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.1% sodium azide, 40% glycerol.
Supplied as a liquid in PBS, pH 7.4 and 0.1% BSA, 0.1% sodium azide, 40% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
749
Applicable Applications for anti-NCKX1 antibody
ELISA, Western Blot
Application Notes
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL.
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml.
Optimal dilutions to be determined by the researcher.
Immunogen
A 21 AA Peptide sequence near the N-terminus of mature human NCKX1 (1). The peptide was coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-NCKX1 antibody
Ca2+ plays a critical role in intracellular signaling. Intracellular Ca2+ levels are tightly controlled by continuos removal of Ca2+ via ATP-driven Ca2+ pump in the endoplasmic reticulum and plasma membrane, and Ca2+ transport system, the Na+/Ca2+ exchangers (NCX), in the plasma membrane. NCX can move Ca2+ either into or out of cells, depending on the net Na+, Ca2+, and K+ gradient across the membrane. In most cells, 3 Na+ are exchanged for 1 Ca2+. In mammals, at least 5 distinct genes code for the exchangers: Three NCX (NCX1, NCX2, and NCX3), and two in the NCKX family (NCKX1 and NCKX2). NCX share significant sequence homology (~70%), display 11 TM domains, a large central, intracellular hydrophilic regulatory loop between TM5 and 6, extracellular N-terminus and cytoplasmic C-terminus. The N-terminal signal peptide is cleaved off from the mature exchanger protein.
Product Categories/Family for anti-NCKX1 antibody
References
• 1). Tucker JE et al (1998) Invest. Opthalmol. 39, 435; Walter MA et al (1998) Human Genet. 103, 411; Poon S et al (1999) Gene accession # AF176688; Reilander H et al (1992) EMBO J. 11, 1689; Kim TSY et al (1998) J Biol. Chem. 273, 16561-16567
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
NCKX1
Similar Products
Product Notes
The NCKX1 (Catalog #AAA610449) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - NCKX1 (K+-dependent, Na Ca Exchanger 1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCKX1 can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure antibody using ECL. ELISA:1:10,000-50,000 for neat serum and 0.5-1ug/ml for affinity purified. Control peptide can be used to coat ELISA plates at 1ug/ml. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the NCKX1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCKX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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