Rabbit NCF2 Polyclonal Antibody | anti-NCF2 antibody

Anti-NCF2 Antibody

Cat. Num. AAA419585

• Gene Names: NCF2; NCF-2; NOXA2; P67PHOX; P67-PHOX
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity Purification
• Synonyms: NCF2; Polyclonal Antibody; Anti-NCF2 Antibody; anti-NCF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of THP1 cell and mouse spleen cell lysate using NCF2 antibody. Immunohistochemistry of paraffin-embedded human esophageal cancer using NCF2 antibody at dilution of 1:100 (400x lens). Immunohistochemistry of paraffin-embedded human esophagus using NCF2 antibody at dilution of 1:100 (400x lens). Immunofluorescence analysis of HeLa cell using NCF2 antibody. Blue: DAPI for nuclear staining.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 481

• Applicable Applications for anti-NCF2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200; ICC: 1:20- 1:100; IP: 1:20- 1:100; IF: 1:50- 1:200
• Immunogen: Recombinant protein of human NCF2
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Related Product Information for anti-NCF2 antibody

The phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The NADPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformational change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558 (1). Defects in p47phox, often resulting from recombination between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease (2-4). Elevated oxidative stress due to increased myocardial NADPH oxidase activity may be a contributing factor in heart failure (5, 6). p67phox appears to coordinate assembly of NAPDH oxidase as it associates with multiple subunits as well as the +/- subunit of heterotrimeric G proteins (7). Mutations in the corresponding p67phox gene are also associated with a form of autosomal recessive chronic granulomatous disease (8).

NCBI and Uniprot Product Information

• NCBI GI #: 299829294
• NCBI GeneID: 4688
• NCBI Accession #: NP_001177723.1
• NCBI GenBank Nucleotide #: NM_001190794.1
• UniProt Accession #: P19878; Q2PP06; Q8NFC7; Q9BV51; B2R6Q1; B4DKQ7; B4DQA7; E9PHJ2; E9PHX3
• NCBI Official Full Name: neutrophil cytosol factor 2 isoform 2
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 2
• NCBI Official Symbol: NCF2
• NCBI Official Synonym Symbols: NCF-2; NOXA2; P67PHOX; P67-PHOX
• NCBI Protein Information: neutrophil cytosol factor 2
• UniProt Protein Name: Neutrophil cytosol factor 2
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF2
• UniProt Synonym Gene Names: NOXA2; P67PHOX; NCF-2

NCBI Description

This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

Uniprot Description

p67phox: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). Interacts with SYTL1 and RAC1. Interacts with NCF4. May interact with NOXO1. Belongs to the NCF2/NOXA1 family.

Protein type: Oxidoreductase

Chromosomal Location of Human Ortholog: 1q25.3

Cellular Component: cytoplasm; cytosol; NADPH oxidase complex; nucleolus

Molecular Function: electron carrier activity; protein binding; protein C-terminus binding

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; cellular defense response; innate immune response; respiratory burst; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type Ii