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Western Blot (WB) (Western blot analysis of NCF1C Antibody (C-term) in T47D cell line lysates (35ug/lane). NCF1C (arrow) was detected using the purified Pab.)

Rabbit anti-Human NCF1C Polyclonal Antibody | anti-NCF1C antibody

NCF1C, CT (Putative Neutrophil Cytosol Factor 1C, NCF-1C, Putative SH3 and PX Domain-containing Protein 1C, SH3PXD1C) (Biotin)

Gene Names
NCF1C; SH3PXD1C
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
NCF1C; Polyclonal Antibody; CT (Putative Neutrophil Cytosol Factor 1C; NCF-1C; Putative SH3 and PX Domain-containing Protein 1C; SH3PXD1C) (Biotin); Neutrophil Cytosolic Factor 1C Pseudogene; anti-NCF1C antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NCF1C.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-NCF1C antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human NCF1C (KLH).
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NCF1C Antibody (C-term) in T47D cell line lysates (35ug/lane). NCF1C (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of NCF1C Antibody (C-term) in T47D cell line lysates (35ug/lane). NCF1C (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCF1C Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCF1C Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NCF1C Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (NCF1C Antibody (C-term) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-NCF1C antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,851 Da
NCBI Official Full Name
Putative neutrophil cytosol factor 1C
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1C pseudogene
NCBI Official Symbol
NCF1C
NCBI Official Synonym Symbols
SH3PXD1C
UniProt Protein Name
Putative neutrophil cytosol factor 1C
UniProt Gene Name
NCF1C
UniProt Synonym Gene Names
SH3PXD1C; NCF-1C

NCBI Description

The neutrophil cytosolic factor 1 (NCF1) gene encodes the 47 kDa cytosolic subunit of neutrophil NADPH oxidase, which produces superoxide anion. The NCF1 gene is located in close proximity to two highly similar, multi-exon pseudogenes at chromosome 7q11.23, corresponding to this gene record and GeneID:654816. The two pseudogenes contain a dinucleotide deletion (delta-GT) in exon 2 that results in a frameshift and truncation of the open reading frame, and neither pseudogene is likely to express a protein. Recombination events between the pseudogenes and the functional NCF1 gene can inactivate the NCF1 gene and result in chronic granulomatous disease. [provided by RefSeq, Nov 2009]

Uniprot Description

May be required for activation of the latent NADPH oxidase (necessary for superoxide production).

Research Articles on NCF1C

Similar Products

Product Notes

The NCF1C ncf1c (Catalog #AAA6489453) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCF1C, CT (Putative Neutrophil Cytosol Factor 1C, NCF-1C, Putative SH3 and PX Domain-containing Protein 1C, SH3PXD1C) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NCF1C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NCF1C ncf1c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCF1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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