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Testing Data #1 (Western blot analysis of U937 cell lysate probed with Goat anti Human NCF1 (MBS221668))

Goat NCF1 Polyclonal Antibody | anti-NCF1 antibody

GOAT ANTI HUMAN NCF1

Gene Names
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Reactivity
Bovine (Expected from Sequence), Dolphin (Expected from Sequence), Mouse (Expected from Sequence), Rabbit (Expected from Sequence), Rat (Expected from Sequence)
Applications
Western Blot
Synonyms
NCF1; Polyclonal Antibody; GOAT ANTI HUMAN NCF1; anti-NCF1 antibody
Ordering
For Research Use Only!
Host
Goat
Reactivity
Bovine (Expected from Sequence), Dolphin (Expected from Sequence), Mouse (Expected from Sequence), Rabbit (Expected from Sequence), Rat (Expected from Sequence)
Clonality
Polyclonal
Isotype
IgG
Specificity
This item recognises neutrophil cytosolic factor 1 (NCF1), a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.
Form/Format
Purified
Purified IgG - liquid
Concentration
IgG concentration 0.5 mg/ml (varies by lot)
Sequence Length
390
Applicable Applications for anti-NCF1 antibody
Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 47kDa in U937 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.
Western Blotting: Minimum Dilution: 0.2ug/ml; Maximum Dilution: 2ug/ml
Perservative Stabilisers
0.02% Sodium Azide
0.5% Bovine Serum Albumin
Immunogen
Peptide sequence CSESTKRKLASAV corresponding to the C-terminus of NCF1 (NP_000256).
Buffer Solution
Antiserum Preparation
Antisera to NCF1 were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of U937 cell lysate probed with Goat anti Human NCF1 (MBS221668))

Testing Data #1 (Western blot analysis of U937 cell lysate probed with Goat anti Human NCF1 (MBS221668))
Related Product Information for anti-NCF1 antibody
MBS221668 recognises neutrophil cytosolic factor 1 (NCF1), a 47kD cytosolic subunit of the multi-protein complex known as NADPH oxidase. Expression of NCF1 is restricted to granulocytes, monocytes and B lymphocytes. Defects in NCF1 have been reported to be a cause of autosomal recessive chronic granulomatous disease.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,781 Da
NCBI Official Full Name
neutrophil cytosol factor 1
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1
NCBI Official Symbol
NCF1
NCBI Official Synonym Symbols
NCF1A; NOXO2; p47phox; SH3PXD1A
NCBI Protein Information
neutrophil cytosol factor 1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NADPH oxidase organizer 2; NCF-1; NCF-47K; SH3 and PX domain-containing protein 1A; neutrophil NADPH oxidase factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); nox organizer 2; nox-organizing protein 2; p47-phox
UniProt Protein Name
Neutrophil cytosol factor 1
Protein Family
UniProt Gene Name
NCF1
UniProt Synonym Gene Names
NOXO2; SH3PXD1A; NCF-1
UniProt Entry Name
NCF1_HUMAN

NCBI Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Uniprot Description

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; extrinsic to membrane; rough endoplasmic reticulum; cell soma; dendrite; cytosol; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding

Biological Process: respiratory burst during defense response; respiratory burst; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; antigen processing and presentation of exogenous peptide antigen via MHC class I; cellular defense response; innate immune response; response to yeast; vascular endothelial growth factor receptor signaling pathway; protein targeting to membrane; inflammatory response; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I

Research Articles on NCF1

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Product Notes

The NCF1 ncf1 (Catalog #AAA221668) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The GOAT ANTI HUMAN NCF1 reacts with Bovine (Expected from Sequence), Dolphin (Expected from Sequence), Mouse (Expected from Sequence), Rabbit (Expected from Sequence), Rat (Expected from Sequence) and may cross-react with other species as described in the data sheet. AAA Biotech's NCF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: This item detects a band of approximately 47kDa in U937 cell lysates. A minimum incubation time of 1 hour is recommended with this antibody. Western Blotting: Minimum Dilution: 0.2ug/ml; Maximum Dilution: 2ug/ml. Researchers should empirically determine the suitability of the NCF1 ncf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NCF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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