Rabbit anti-Human Natural Resistance Associated Macrophage Protein Polyclonal Antibody | anti-Slc11a1 antibody

Natural Resistance Associated Macrophage Protein (Nramp2)

Cat. Num. AAA614863

• Gene Names: Slc11a1; Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Natural Resistance Associated Macrophage Protein; Polyclonal Antibody; Natural Resistance Associated Macrophage Protein (Nramp2); Anti -Natural Resistance Associated Macrophage Protein (Nramp2); anti-Slc11a1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Natural Resistance Associated Macrophage Protein (Nramp2). Species sequence homology: monkey 88%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophillized powder in PBS, pH 7.4, 0.1% BSA. Reconstitute with 100ul sterile PBS.

• Applicable Applications for anti-Slc11a1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry and Western Blot.; Dilution: Western Blot: 1-10ug/ml using ECL.; ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.; Immunohistochemistry: 5-20ug/ml.
• Immunogen: A 17 AA Peptide sequence located at the predicted cytoplasmic loop near the C-terminus of human NRAMP2, with IRE (1). Coupled to KLH.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Slc11a1 antibody

Natural resistance to infection with unrelated intracellular parasite such as Mycobacteria, Salmonella, and Leishmania is controlled by a single gene that encodes a macrophage-specific membrane protein designated as Natural Resistance-Associated Macrophage Protein (Nramp1). Recently a second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568 aa, ~65% identity with NRAMP1). Unlike NRAMP1, NRAMP2 expression is more ubiquitous and has been detected in most tissues. It is dramatically up-regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). Interestingly, one Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18-aa of the IRE form (with IRE, isoform I) are replaced by a novel 25-aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has recently been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgarde (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.

Product Categories/Family for anti-Slc11a1 antibody

Antibodies; Abs to Ion Transport

NCBI and Uniprot Product Information

• NCBI GI #: 505156
• NCBI GeneID: 18173
• UniProt Accession #: P41251; Q3TB84
• Molecular Weight: 59,741 Da
• NCBI Official Full Name: natural resistance associated macrophage protein
• NCBI Official Synonym Full Names: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
• NCBI Official Symbol: Slc11a1
• NCBI Official Synonym Symbols: Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
• NCBI Protein Information: natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh
• UniProt Protein Name: Natural resistance-associated macrophage protein 1
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: Slc11a1
• UniProt Synonym Gene Names: Bcg; Ity; Lsh; Nramp1
• UniProt Entry Name: NRAM1_MOUSE

Uniprot Description

SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Membrane protein, integral; Cell surface; Transporter, SLC family; Membrane protein, multi-pass; Transporter; Vesicle

Cellular Component: phagocytic vesicle membrane; cell surface; membrane; lysosome; late endosome; plasma membrane; integral to membrane; vacuole; endosome membrane

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; transporter activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; wound healing; positive regulation of T-helper 1 type immune response; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; antigen processing and presentation of peptide antigen; vacuolar acidification; nitrite transport; transport; T cell proliferation during immune response; T cell cytokine production; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; positive regulation of cytokine production; mRNA stabilization; MAPKKK cascade; L-arginine import; defense response to protozoan; iron ion transport; interleukin-2 production; phagocytosis; iron ion homeostasis; positive regulation of interferon-gamma production; MHC class II biosynthetic process; macrophage activation; cellular cadmium ion homeostasis; response to bacterium; protein import into nucleus, translocation; negative regulation of cytokine production; defense response to bacterium; activation of protein kinase activity; ion transport; positive regulation of transcription from RNA polymerase II promoter