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Western Blot (WB) (Western blot analysis of NAT13 expression in SKOV3 (A), MCF7 (B), mouse brain (C) whole cell lysates.)

Rabbit NAT13 Polyclonal Antibody | anti-NAA50 antibody

Anti-NAT13 Antibody

Gene Names
NAA50; SAN; MAK3; NAT5; NAT13; NAT5P; NAT13P
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry
Purity
The antibody was purified by immunogen affinity chromatography.
Synonyms
NAT13; Polyclonal Antibody; Anti-NAT13 Antibody; MAK3; NAT5; N-alpha-acetyltransferase 50; N-acetyltransferase 13; N-acetyltransferase 5; hNAT5; N-acetyltransferase san homolog; hSAN; NatE catalytic subunit; anti-NAA50 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of NAT13 protein.
Purity/Purification
The antibody was purified by immunogen affinity chromatography.
Form/Format
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Sequence Length
81
Applicable Applications for anti-NAA50 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200
Immunogen
Recombinant full length protein of human NAT13
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of NAT13 expression in SKOV3 (A), MCF7 (B), mouse brain (C) whole cell lysates.)

Western Blot (WB) (Western blot analysis of NAT13 expression in SKOV3 (A), MCF7 (B), mouse brain (C) whole cell lysates.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of NAT13 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of NAT13 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

Immunofluorescence (IF)

(Immunofluorescent analysis of NAT13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

Immunofluorescence (IF) (Immunofluorescent analysis of NAT13 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Related Product Information for anti-NAA50 antibody
Rabbit polyclonal antibody to NAT13

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,465 Da
NCBI Official Full Name
N-alpha-acetyltransferase 50 isoform 1
NCBI Official Synonym Full Names
N(alpha)-acetyltransferase 50, NatE catalytic subunit
NCBI Official Symbol
NAA50
NCBI Official Synonym Symbols
SAN; MAK3; NAT5; NAT13; NAT5P; NAT13P
NCBI Protein Information
N-alpha-acetyltransferase 50
UniProt Protein Name
N-alpha-acetyltransferase 50
Protein Family
UniProt Gene Name
NAA50
UniProt Synonym Gene Names
MAK3; NAT13; NAT5; hNAT5; hSAN
UniProt Entry Name
NAA50_HUMAN

Uniprot Description

NAA50: Probable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity. Belongs to the acetyltransferase family. GNAT subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.-; Acetyltransferase

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasm

Molecular Function: H4 histone acetyltransferase activity; peptide alpha-N-acetyltransferase activity; protein binding

Biological Process: N-terminal protein amino acid acetylation

Research Articles on NAA50

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Product Notes

The NAA50 naa50 (Catalog #AAA8245739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NAT13 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NAT13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1/500 - 1/2000; IHC: 1/50 - 1/200; IF/ICC: 1/50 - 1/200. Researchers should empirically determine the suitability of the NAA50 naa50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT13, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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