Western Blot (WB)
(NAT12 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NAT12 antibody detected the NAT12 protein (arrow).)
Rabbit anti-Mouse NAT12 Polyclonal Antibody | anti-NAT12 antibody
NAT12, CT (NAA30, C14orf35, MAK3, NAT12, N-alpha-acetyltransferase 30, N-acetyltransferase 12, N-acetyltransferase MAK3 homolog, NatC catalytic subunit) (PE)
Gene Names
NAA30; MAK3; Mak3p; NAT12; NAT12P; C14orf35
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
NAT12; Polyclonal Antibody; CT (NAA30; C14orf35; MAK3; N-alpha-acetyltransferase 30; N-acetyltransferase 12; N-acetyltransferase MAK3 homolog; NatC catalytic subunit) (PE); NAA30; NatC catalytic subunit; anti-NAT12 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-NAT12 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
NAT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 322-350 amino acids from the C-terminal region of human NAT12.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(NAT12 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NAT12 antibody detected the NAT12 protein (arrow).)
Western Blot (WB)
(NAT12 Antibody (C-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the NAT12 antibody detected the NAT12 protein (arrow).)
Immunohistochemistry (IHC)
(NAT12 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAT12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(NAT12 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAT12 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Product Categories/Family for anti-NAT12 antibody
References
Polevoda, B., et al. BMC Proc 3 SUPPL 6, S2 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,580 Da
NCBI Official Full Name
N-alpha-acetyltransferase 30
NCBI Official Synonym Full Names
N(alpha)-acetyltransferase 30, NatC catalytic subunit
NCBI Official Symbol
NAA30
NCBI Official Synonym Symbols
MAK3; Mak3p; NAT12; NAT12P; C14orf35
NCBI Protein Information
N-alpha-acetyltransferase 30; N-acetyltransferase 12 (GCN5-related, putative); N-acetyltransferase MAK3 homolog; N-alpha-acetyltransferase 30, NatC catalytic subunit; natC catalytic subunit; putative N-acetyltransferase
UniProt Protein Name
N-alpha-acetyltransferase 30
Protein Family
UniProt Gene Name
NAA30
UniProt Synonym Gene Names
C14orf35; MAK3; NAT12
UniProt Entry Name
NAA30_HUMAN
Uniprot Description
NAA30: Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. Necessary for the lysosomal localization and function of ARL8B. Belongs to the acetyltransferase family. MAK3 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Acetyltransferase; EC 2.3.1.88
Chromosomal Location of Human Ortholog: 14q22.3
Cellular Component: polysome; cytoplasm
Molecular Function: peptide alpha-N-acetyltransferase activity; protein binding
Biological Process: N-terminal protein amino acid acetylation; N-terminal peptidyl-methionine acetylation
Research Articles on NAT12
Similar Products
Product Notes
The NAT12 naa30 (Catalog #AAA6323901) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NAT12, CT (NAA30, C14orf35, MAK3, NAT12, N-alpha-acetyltransferase 30, N-acetyltransferase 12, N-acetyltransferase MAK3 homolog, NatC catalytic subunit) (PE) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAT12 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:10-50 FLISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the NAT12 naa30 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAT12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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