Rabbit anti-Mouse NaPi-2B Polyclonal Antibody | anti-Slc9a3r1 antibody

NaPi-2B (Npt IIb)

Cat. Num. AAA612158

• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: NaPi-2B; Polyclonal Antibody; NaPi-2B (Npt IIb); Anti -NaPi-2B (Npt IIb); anti-Slc9a3r1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse NaPi-2B (Npt IIb). No significant sequence homology is observed with NaPi-2a or other Pi-transporters (Type-I, III or IV) transporters.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA.

• Applicable Applications for anti-Slc9a3r1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Immunogen: Synthetic peptide corresponding to15aa within the cytoplasmic, C-terminus of mouse NaPi-2B conjugated to KLH. Species Homology: rat 93%.
• Preparation and Storage: -20 degree C

Related Product Information for anti-Slc9a3r1 antibody

Inorganic phosphate (Pi) levels are hormonally regulated, affecting the physiological activity of bone, kidney and small intestine. Majority of the Pi is absorbed in the small intestine and reabsorbed in the proximal tubules in the kidney. At least 4 groups of structurally and functionally related proteins are involved in Pi transport: Type I-related NaPi transporters designated NPT1, Npt1 and NaPi-1, respectively, in humans, mouse and rabbit, are expressed in the kidney and liver. Its expression and activity are not regulated by Pi deprivation or parathyroid hormone (PTH) and its role in Pi-homeostasis is not clear. Type IIa-related cotransporters, designated NaPi-2 in rat, NaPi-3 or NPT2 in humans, NaPi-4 in opossum, NaPi-5 in flounder vessel, NaPi-6 or Npt-2 in mouse, and NaPi-7 in rabbit, is the primary target for Pi regulation by dietary, hormonal and tubular Pi reabsorption. Deletion of Npt2 gene produces severe Pi wasting. Type II transporters are expressed in kidney, brain, lung, bone and small intestine. Type IIb, designated as NaPi-IIB or NaPi-2b in rat/mouse, and NaPi-3b in human, is closely related isoform of the NaPi-2 family. It is expressed in small intestine and lung. Type III NaPi transporters, originally described as a family of cell surface receptors for gibbon ape leukemia virus (GALV) and murine amphotropic retrovirus (A-MuLv), share very low (< 20%) sequence homology with Type I and II proteins, and are found in most tissues. Human homolog is now designated as PiT1 (also known as GALV receptors or GLVR1) and PiT2 (A-MuLV receptors or GLVR2 or Ram1). Pit1 and Pit2 are ~62% related and predicted to contain 10 TM domains that is in contrast with Type 1/2 transporters (6-8 TM). The N- and C-termini are predicted to be cytoplasmic.

Product Categories/Family for anti-Slc9a3r1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 11024674
• NCBI GeneID: 59114
• NCBI Accession #: NP_067605.1
• NCBI GenBank Nucleotide #: NM_021594.1
• UniProt Accession #: Q9JJ19
• Molecular Weight: 38,830 Da
• NCBI Official Full Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• NCBI Official Synonym Full Names: solute carrier family 9, subfamily A (NHE3, cation proton antiporter 3), member 3 regulator 1
• NCBI Official Symbol: Slc9a3r1
• NCBI Protein Information: Na(+)/H(+) exchange regulatory cofactor NHE-RF1; Na(+)/H(+) exchange regulatory cofactor NHE-RF1; EBP50; NHERF-1; ERM-binding phosphoprotein; regulatory cofactor of Na(+)/H(+) exchanger; sodium-hydrogen exchanger regulatory factor 1; ezrin-radixin-moesin-binding phosphoprotein 50; solute carrier family 9 isoform A3 regulatory factor 1; solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1; solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1
• UniProt Protein Name: Na(+)/H(+) exchange regulatory cofactor NHE-RF1
• Protein Family: Na(+)/H(+) exchange regulatory cofactor
• UniProt Gene Name: Slc9a3r1
• UniProt Synonym Gene Names: Nherf; Nherf1; NHERF-1; EBP50
• UniProt Entry Name: NHRF1_RAT

NCBI Description

a membrane-cytoskeleton linking protein thought to be involved in sequestering and regulating the activity of integral membrane proteins, and in modulating membrane protein trafficking [RGD, Feb 2006]

Uniprot Description

NHERF: a scaffold protein that connects plasma membrane proteins with members of the Ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized beta-2-adrenergic receptor Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. May enhance Wnt signaling.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Cellular Component: centrosome; microvillus; intracellular membrane-bound organelle; cell; brush border membrane; endomembrane system; stereocilium bundle tip; lipid raft; ruffle; stereocilium; membrane; microvillus membrane; apical part of cell; apical plasma membrane; cytoplasm; plasma membrane; intracellular; nucleus; vesicle; filopodium

Molecular Function: protein domain specific binding; protein binding; chloride channel regulator activity; protein self-association; beta-2 adrenergic receptor binding; protein complex binding; beta-catenin binding; dopamine receptor binding; protein complex scaffold; receptor binding; phosphatase binding; PDZ domain binding

Biological Process: bile acid secretion; negative regulation of protein kinase B signaling cascade; Wnt receptor signaling pathway; renal sodium ion transport; negative regulation of cell proliferation; microvillus biogenesis; cAMP-mediated signaling; cellular phosphate ion homeostasis; sensory perception of sound; dopamine receptor, phospholipase C activating pathway; auditory receptor cell stereocilium organization and biogenesis; actin cytoskeleton organization and biogenesis; negative regulation of sodium:hydrogen antiporter activity; regulation of protein kinase activity; negative regulation of cell migration; dopamine receptor, adenylate cyclase activating pathway; negative regulation of phosphoinositide 3-kinase cascade