Rabbit NALP12 Polyclonal Antibody | anti-NLRP12 antibody

NALP12 Antibody

Cat. Num. AAA151244

• Gene Names: NLRP12; RNO; PAN6; RNO2; FCAS2; NALP12; PYPAF7; CLR19.3
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: NALP12 Antibody is affinity chromatography purified via peptide column.
• Synonyms: NALP12; Polyclonal Antibody; NALP12 Antibody; RNO; PAN6; RNO2; FCAS2; PYPAF7; CLR19.3; NACHT; LRR and PYD domains-containing protein 12; Monarch-1; NLR family; pyrin domain containing 12; anti-NLRP12 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: NALP12 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1061

• Applicable Applications for anti-NLRP12 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: NALP12 antibody can be used for detection of NALP12 by Western blot at 1 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NALP12 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human NALP12.
• Buffer: NALP12 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NALP12 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NALP12 in human brain tissue lysate with NALP12 antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of NALP12 in human brain tissue with NALP12 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NALP12 in human brain tissue with NALP12 antibody at 20 μg/mL.)

Related Product Information for anti-NLRP12 antibody

NALP12 Antibody: NALP proteins are cytoplasmic proteins that form a subfamily within the larger CATERPILLER family and are thought to play a crucial role in cell proliferation and reproduction. Like all other NALP family members, NALP12, also known as Monarch-1, has a C-terminal leucine-rich repeat (LRR) region, an N-terminal Pyrin domain (PYD) followed by a NACHT domain, and a NACHT-associated domain. NALP12 is thought to act as an attenuating factor of inflammation by suppressing inflammatory responses such as NF-κB activation by TLR-signaling molecules MyD88, IRAK-1, TRAF6 and RIPK1 in activated monocytes. Recent evidence suggests that mutations in NALP12 result in hereditary periodic fever syndromes.

NCBI and Uniprot Product Information

• NCBI GI #: 21955154
• NCBI GeneID: 91662
• NCBI Accession #: NP_653288
• NCBI GenBank Nucleotide #: NM_144687.3
• UniProt Accession #: P59046; Q8NEU4; Q9BY26; A8MTQ2; B3KTE7
• Molecular Weight: 120,329 Da
• NCBI Official Full Name: NACHT, LRR and PYD domains-containing protein 12 isoform 2
• NCBI Official Synonym Full Names: NLR family, pyrin domain containing 12
• NCBI Official Symbol: NLRP12
• NCBI Official Synonym Symbols: RNO; PAN6; RNO2; FCAS2; NALP12; PYPAF7; CLR19.3
• NCBI Protein Information: NACHT, LRR and PYD domains-containing protein 12; monarch 1; regulated by nitric oxide; PYRIN-containing APAF1-like protein 7; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 12
• UniProt Protein Name: NACHT, LRR and PYD domains-containing protein 12
• Protein Family: NACHT, LRR and PYD domains-containing protein
• UniProt Gene Name: NLRP12
• UniProt Synonym Gene Names: NALP12; PYPAF7; RNO
• UniProt Entry Name: NAL12_HUMAN

NCBI Description

This gene encodes a member of the CATERPILLER family of cytoplasmic proteins. The encoded protein, which contains an N-terminal pyrin domain, a NACHT domain, a NACHT-associated domain, and a C-terminus leucine-rich repeat region, functions as an attenuating factor of inflammation by suppressing inflammatory responses in activated monocytes. Mutations in this gene cause familial cold autoinflammatory syndrome type 2. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]

Uniprot Description

NLRP12: May mediate activation of CASP1 via ASC and promote activation of NF-kappa-B via IKK. Defects in NLRP12 are the cause of familial cold autoinflammatory syndrome type 2 (FCAS2). FCAS are rare autosomal dominant systemic inflammatory diseases characterized by episodes of rash, arthralgia, fever and conjunctivitis after generalized exposure to cold. Belongs to the NLRP family. 5 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; caspase activator activity; ATP binding

Biological Process: release of cytoplasmic sequestered NF-kappaB; caspase activation; negative regulation of cytokine secretion; negative regulation of interleukin-6 biosynthetic process; negative regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-1 beta secretion; signal transduction; regulation of caspase activity; negative regulation of signal transduction; negative regulation of Toll signaling pathway; inhibition of NF-kappaB transcription factor; negative regulation of inflammatory response; negative regulation of protein amino acid autophosphorylation; positive regulation of MHC class I biosynthetic process; regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of interleukin-1 secretion; regulation of interleukin-18 biosynthetic process; positive regulation of inflammatory response

Disease: Familial Cold Autoinflammatory Syndrome 2