Rabbit anti-Human NAIP Polyclonal Antibody | anti-NAIP antibody

NAIP Antibody

Cat. Num. AAA151490

• Gene Names: NAIP; BIRC1; NLRB1; psiNAIP
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: NAIP Antibody is affinity chromatography purified via peptide column.
• Synonyms: NAIP; Polyclonal Antibody; NAIP Antibody; BIRC1; NLRB1; psiNAIP; Baculoviral IAP repeat-containing protein 1; Neuronal apoptosis inhibitory protein; NLR family; apoptosis inhibitory protein; anti-NAIP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Minor, lower molecular weight bands may represent alternately spliced forms.
• Purity/Purification: NAIP Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1403

• Applicable Applications for anti-NAIP antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: NAIP antibody can be used for the detection of NAIP by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: NAIP antibody was raised against a synthetic peptide corresponding to 13 amino acids at the C-terminus of human NAIP.
• Buffer: NAIP Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: NAIP antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of NAIP in human tonsil tissue lysate with NAIP antibody at 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of NAIP in A549 cells with NAIP antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of NAIP in A549 cells with NAIP antibody at 20 μg/mL.)

Related Product Information for anti-NAIP antibody

NAIP Antibody: Neuronal apoptosis inhibitor protein (NAIP) was the first human inhibitor of apoptosis protein (IAP) identified and was discovered by its association with the neurodegenerative disorder spinal muscular atrophy. Members of the IAP family contain one to three copies of an approximately 70 amino acid motif termed baculovirus IAP repeat (BIR); these BIRs promote protein-protein interactions with various caspases such as caspase-3, -7, and -9 as well as members of the TRAF family of signal molecules. Unlike other IAPs however, NAIP requires ATP to bind caspase-9 and is not inhibited by the IAP-inhibiting molecule Smac/DIABLO, suggesting that NAIP is unique among the IAPs in its regulation of its activity. Finally, although only one human NAIP protein has been identified, other shorter NAIP mRNA transcripts have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 1737213
• NCBI GeneID: 4671
• UniProt Accession #: Q13075; O75857; Q13730; Q59GI6; Q8TDZ4; Q99796; B9EG72; E9PHD1
• Molecular Weight: 141,329 Da
• NCBI Official Full Name: neuronal apoptosis inhibitory protein
• NCBI Official Synonym Full Names: NLR family, apoptosis inhibitory protein
• NCBI Official Symbol: NAIP
• NCBI Official Synonym Symbols: BIRC1; NLRB1; psiNAIP
• NCBI Protein Information: baculoviral IAP repeat-containing protein 1; neuronal apoptosis inhibitory protein; psi neuronal apoptosis inhibitory protein; nucleotide-binding oligomerization domain, leucine rich repeat and BIR domain containing 1
• UniProt Protein Name: Baculoviral IAP repeat-containing protein 1
• Protein Family: Putative niacin/nicotinamide transporter
• UniProt Gene Name: NAIP
• UniProt Synonym Gene Names: BIRC1
• UniProt Entry Name: BIRC1_HUMAN

NCBI Description

This gene is part of a 500 kb inverted duplication on chromosome 5q13. This duplicated region contains at least four genes and repetitive elements which make it prone to rearrangements and deletions. The repetitiveness and complexity of the sequence have also caused difficulty in determining the organization of this genomic region. This copy of the gene is full length; additional copies with truncations and internal deletions are also present in this region of chromosome 5q13. It is thought that this gene is a modifier of spinal muscular atrophy caused by mutations in a neighboring gene, SMN1. The protein encoded by this gene contains regions of homology to two baculovirus inhibitor of apoptosis proteins, and it is able to suppress apoptosis induced by various signals. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

BIRC1: Anti-apoptotic protein which acts by inhibiting the activities of CASP3, CASP7 and CASP9. Can inhibit the autocleavage of pro-CASP9 and cleavage of pro-CASP3 by CASP9. Capable of inhibiting CASP9 autoproteolysis at 'Asp-315' and decreasing the rate of auto proteolysis at 'Asp-330'. Acts as a mediator of neuronal survival in pathological conditions. Prevents motor- neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.

Chromosomal Location of Human Ortholog: 5q13.2

Cellular Component: spindle microtubule; basolateral plasma membrane; cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; caspase inhibitor activity; ubiquitin-protein ligase activity; ATP binding

Biological Process: nervous system development; innate immune response; protein ubiquitination; regulation of signal transduction; negative regulation of caspase activity; negative regulation of neuron apoptosis; inflammatory response; negative regulation of apoptosis