Rabbit anti-Human NAE1 Polyclonal Antibody | anti-NAE1 antibody

NAE1, ID (NAE1, APPBP1, NEDD8-activating enzyme E1 regulatory subunit, Amyloid beta precursor protein-binding protein 1, 59kD, Amyloid protein-binding protein 1, Proto-oncogene protein 1) (APC)

Cat. Num. AAA6323661

• Gene Names: NAE1; HPP1; ula-1; APPBP1; A-116A10.1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NAE1; Polyclonal Antibody; ID (NAE1; APPBP1; NEDD8-activating enzyme E1 regulatory subunit; Amyloid beta precursor protein-binding protein 1; 59kD; Amyloid protein-binding protein 1; Proto-oncogene protein 1) (APC); Proto-oncogene protein 1; anti-NAE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
• Sequence Length: 445

• Applicable Applications for anti-NAE1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: NAE1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human NAE1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(NAE1 Antibody (Center) western blot analysis in K562,293 cell line lysates (35ug/lane).This demonstrates the NAE1 antibody detected the NAE1 protein (arrow).)

Immunohistochemistry (IHC)

(NAE1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of NAE1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-NAE1 antibody

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-NAE1 antibody

Antibodies; Amyloid

References

Jung, A.C., et al. Int. J. Cancer 126(8):1882-1894(2010). Park, Y., et al. Biochem. Biophys. Res. Commun. 374(2):294-298(2008). Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Li, T., et al. Arch. Biochem. Biophys. 453(1):70-74(2006). Norman, J.A., et al. Biochemistry 45(9):3014-3019(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 66363688
• NCBI GeneID: 8883
• NCBI Accession #: NP_001018170.1; NP_003896.1
• NCBI GenBank Nucleotide #: NP_001018170.1
• UniProt Accession #: Q13564
• NCBI Official Full Name: NEDD8-activating enzyme E1 regulatory subunit isoform c
• NCBI Official Synonym Full Names: NEDD8 activating enzyme E1 subunit 1
• NCBI Official Symbol: NAE1
• NCBI Official Synonym Symbols: HPP1; ula-1; APPBP1; A-116A10.1
• NCBI Protein Information: NEDD8-activating enzyme E1 regulatory subunit
• UniProt Protein Name: NEDD8-activating enzyme E1 regulatory subunit
• Protein Family: NEDD8-activating enzyme E1 regulatory
• UniProt Gene Name: NAE1
• UniProt Synonym Gene Names: APPBP1; APP-BP1
• UniProt Entry Name: ULA1_HUMAN

NCBI Description

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

APPBP1: Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation. Belongs to the ubiquitin-activating E1 family. ULA1 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 16q22

Cellular Component: cytoplasm; plasma membrane; nucleus; cytosol

Molecular Function: NEDD8 activating enzyme activity; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding

Biological Process: regulation of apoptosis; neuron apoptosis; protein neddylation; regulation of neuron apoptosis; mitotic cell cycle DNA replication checkpoint; signal transduction