Western Blot (WB)
(Western blot analysis on HuvEc cell lysate using NADAP Antibody, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Mouse NADAP Polyclonal Antibody | anti-NADAP antibody
NADAP Antibody
Gene Names
SLC4A1AP; HLC3
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
NADAP; Polyclonal Antibody; NADAP Antibody; FLJ10624; FLJ41004; HLC-3; HLC3; Human lung cancer oncogene 3 protein; Kanadaptin; Kidney anion exchanger adapter protein; Kidney anion exchanger adaptor protein; Lung cancer oncogene 3 protein; MGC120646; MGC120648; NADAP_HUMAN; SLC4A1AP; Solute carrier family 4 (anion exchanger) member 1 adaptor protein; Solute carrier family 4 anion exchanger member 1 adapter protein; anti-NADAP antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
NADAP antibody detects endogenous levels of NADAP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
796
Applicable Applications for anti-NADAP antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human NADAP
Subcellular Location
Nucleus. Cytoplasm. Mainly nuclear. Small amounts are found in the cytoplasm.
Tissue Specificity
Ubiquitously expressed.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis on HuvEc cell lysate using NADAP Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on HuvEc cell lysate using NADAP Antibody, The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF)
(MBS9600361 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9600361 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-NADAP antibody
Function: PubMed:15764369 initially suggested a role in targeting SLC4A1 (kidney anion exchanger 1) to the plasma membrane; it does not seem to do so as it does not interact with SLC4A1 and has no effect on SLC4A1 trafficking.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 85 kDa
Predicted: 89 kDa
Predicted: 89 kDa
NCBI Official Full Name
kanadaptin
NCBI Official Synonym Full Names
solute carrier family 4 member 1 adaptor protein
NCBI Official Symbol
SLC4A1AP
NCBI Official Synonym Symbols
HLC3
NCBI Protein Information
kanadaptin
UniProt Protein Name
Kanadaptin
UniProt Gene Name
SLC4A1AP
UniProt Synonym Gene Names
HLC-3
Uniprot Description
CautionPubMed:15764369 initially suggested a role in targeting SLC4A1 (kidney anion exchanger 1) to the plasma membrane; it does not seem to do so as it does not interact with SLC4A1 and has no effect on SLC4A1 trafficking.
Research Articles on NADAP
Similar Products
Product Notes
The NADAP slc4a1ap (Catalog #AAA9600361) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NADAP Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NADAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:2000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the NADAP slc4a1ap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NADAP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
