Testing Data
(Anti-SIRT2 Picoband antibody, MBS177451-1.jpgAll lanes: Anti SIRT2 (MBS177451) at 0.5ug/mlWB: Recombinant Human SIRT2 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )
Rabbit NAD-dependent protein deacetylase sirtuin-2 Polyclonal Antibody | anti-SIRT2 antibody
Anti-SIRT2 Antibody
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat
Testing Data
(Anti-SIRT2 Picoband antibody, MBS177451-1.jpgAll lanes: Anti SIRT2 (MBS177451) at 0.5ug/mlWB: Recombinant Human SIRT2 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )
Testing Data
(Anti-SIRT2 Picoband antibody, MBS177451-1.jpgAll lanes: Anti SIRT2 (MBS177451) at 0.5ug/mlWB: Recombinant Human SIRT2 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD )
Testing Data
(Anti-SIRT2 Picoband antibody, MBS177451-2.jpgAll lanes: Anti SIRT2 (MBS177451) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugPredicted bind size: 43KDObserved bind size: 43KD )
Testing Data
(Anti-SIRT2 Picoband antibody, MBS177451-2.jpgAll lanes: Anti SIRT2 (MBS177451) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugLane 3: A549 Whole Cell Lysate at 40ugPredicted bind size: 43KDObserved bind size: 43KD )
Immunohistochemistry (IHC)
(Anti-SIRT2 Picoband antibody, MBS177451-3.JPGIHC(P): Mouse Brain Tissue )
Immunohistochemistry (IHC)
(Anti-SIRT2 Picoband antibody, MBS177451-3.JPGIHC(P): Mouse Brain Tissue )
Immunohistochemistry (IHC)
(Anti-SIRT2 Picoband antibody, MBS177451-4.JPGIHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-SIRT2 Picoband antibody, MBS177451-4.JPGIHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC)
(Anti-SIRT2 Picoband antibody, MBS177451-5.JPGIHC(P): Human Lung Cancer Tissue )
Immunohistochemistry (IHC)
(Anti-SIRT2 Picoband antibody, MBS177451-5.JPGIHC(P): Human Lung Cancer Tissue )
Background: SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT2 is mapped to 19q13.2. It is able to cause radioactivity to be transferred from (32P) NAD to bovine serum albumin (BSA). According to researches, SIRT2 can act as a bona fide tubulin deacetylase. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo. SIRT2 is also a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. What's more, SIRT2 is an important regulator of programmed necrosis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
SIRT2: NAD-dependent protein deacetylase, which deacetylates internal lysines on histone and non-histone proteins. Deacetylates 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. Deacetylates PCK1, opposing proteasomal degradation. Deacetylates 'Lys-310' of RELA. Interacts with HDAC6, suggesting that these proteins belong to a large complex that deacetylate the cytoskeleton. Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. Inhibited by Sirtinol, A3 and M15 small molecules. Inhibited by nicotinamide. Belongs to the sirtuin family. Class I subfamily. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.1.-; Deacetylase
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: centrosome; microtubule; lateral loop; paranode region of axon; perikaryon; chromosome; nuclear heterochromatin; cytosol; centriole; chromatin silencing complex; growth cone; perinuclear region of cytoplasm; cytoplasm; plasma membrane; spindle; midbody; nucleus; myelin sheath
Molecular Function: histone acetyltransferase binding; protein binding; ubiquitin binding; zinc ion binding; tubulin deacetylase activity; NAD-dependent histone deacetylase activity; histone deacetylase binding; protein deacetylase activity; beta-tubulin binding; NAD-dependent histone deacetylase activity (H4-K16 specific); chromatin binding; histone deacetylase activity; transcription factor binding; NAD+ ADP-ribosyltransferase activity
Biological Process: chromatin silencing at rDNA; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of autophagy; phosphoinositide 3-kinase cascade; regulation of myelination; regulation of cell cycle; chromatin silencing; positive regulation of attachment of spindle microtubules to kinetochore; response to redox state; negative regulation of transcription from RNA polymerase II promoter; gene silencing; chromatin silencing at telomere; negative regulation of cell proliferation; substantia nigra development; meiotic cell cycle; protein amino acid deacetylation; cellular lipid catabolic process; protein kinase B signaling cascade; positive regulation of DNA binding; mitosis; regulation of phosphorylation; transcription, DNA-dependent; positive regulation of meiosis; regulation of exit from mitosis; histone deacetylation; hepatocyte growth factor receptor signaling pathway; negative regulation of fat cell differentiation; negative regulation of striated muscle development; myelination in the peripheral nervous system; protein amino acid ADP-ribosylation; cell division; positive regulation of cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; autophagy; innate immune response; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative regulation of protein catabolic process
Research Articles on SIRT2
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Product Notes
The SIRT2 sirt2 (Catalog #AAA177451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT2 Antibody reacts with Human, Mouse, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's NAD-dependent protein deacetylase sirtuin-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC-P). Western Blot: Concentration: 0.1-0.5mug/ml; Tested Species: Human, Rat Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1mug/ml; Tested Species: Human, Mouse, Rat. Researchers should empirically determine the suitability of the SIRT2 sirt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAD-dependent protein deacetylase sirtuin-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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