Goat anti-Human NAALADase-Like 2 Polyclonal Antibody | anti-NAALADL1 antibody
NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2, NAALADL2)
Gene Names
NAALADL1; I100; NAALADASEL
Reactivity
Human
Applications
ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
NAALADase-Like 2; Polyclonal Antibody; NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2; NAALADL2); Anti -NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2; anti-NAALADL1 antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NAALADL2.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS with 5% trehalose. Reconstitute with 200ul 40-50% glycerol, PBS.
Applicable Applications for anti-NAALADL1 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation.
Dilution: Flow Cytometry: use 10ul to label 10e6 cells.
Direct ELISA: 0.5 -1ug/mL detection limit for rhNAALADL2 ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhNAALADL2 ~ 10ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/mL
Dilution: Flow Cytometry: use 10ul to label 10e6 cells.
Direct ELISA: 0.5 -1ug/mL detection limit for rhNAALADL2 ~ 0.3ng/well.
Western Blot: 0.1-0.2ug/ml detection limit for rhNAALADL2 ~ 10ng/lane under non-reducing and reducing conditions.
Immunocytochemistry: 10ug/mL
Immunogen
NS0-derived, recombinant human NAALADL2. (aa 152 - 795)
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-NAALADL1 antibody
NAALADL2 (N-acetylated a-linked acidic dipeptidase like 2) is a member of the peptidase M28 family of enzymes. It is presumably a type II transmembrane (TM) protein that may have O-glycosyl hydrolase activity. Human NAALADL2 is 795 amino acids (aa) in length. It is believed to contain a cytoplasmic and TM segment at the N-terminus, followed by a peptidase domain (aa 444-596) and a TfR-like dimerization region (aa 688-777). There are multiple splice forms. One shows a 4 aa substitution for aa 292-795, a second shows an alternate start site at Met18 with a 24 aa substitution for aa 314-795, and a third contains an alternate start site at
Product Categories/Family for anti-NAALADL1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
80,558 Da
NCBI Official Full Name
N-acetylated-alpha-linked acidic dipeptidase-like protein
NCBI Official Synonym Full Names
N-acetylated alpha-linked acidic dipeptidase-like 1
NCBI Official Symbol
NAALADL1
NCBI Official Synonym Symbols
I100; NAALADASEL
NCBI Protein Information
N-acetylated-alpha-linked acidic dipeptidase-like protein; NAALADase L; ileal peptidase I100; ileal dipeptidylpeptidase; 100 kDa ileum brush border membrane protein
UniProt Protein Name
N-acetylated-alpha-linked acidic dipeptidase-like protein
Protein Family
UniProt Gene Name
NAALADL1
UniProt Synonym Gene Names
NAALADASEL; NAALADL; NAALADase L; I100
UniProt Entry Name
NALDL_HUMAN
Uniprot Description
NAALADL1: NAALADase-like activity unknown. Has no NAAG hydrolyzing activity. Exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. Belongs to the peptidase M28 family. M28B subfamily. 8 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; EC 3.4.17.21; Protease
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: apical plasma membrane; integral to membrane
Molecular Function: dipeptidase activity; peptidase activity; carboxypeptidase activity; metallopeptidase activity; metal ion binding
Biological Process: proteolysis
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Product Notes
The NAALADL1 naaladl1 (Catalog #AAA618050) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The NAALADase-Like 2 (N-acetylated A-linked Acidic Dipeptidase Like 2, NAALADL2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NAALADase-Like 2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Direct ELISA, Western Blot, Immunocytochemistry and Immunoprecipitation. Dilution: Flow Cytometry: use 10ul to label 10e6 cells. Direct ELISA: 0.5 -1ug/mL detection limit for rhNAALADL2 ~ 0.3ng/well. Western Blot: 0.1-0.2ug/ml detection limit for rhNAALADL2 ~ 10ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10ug/mL. Researchers should empirically determine the suitability of the NAALADL1 naaladl1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAALADase-Like 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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