Rabbit anti-Human NAAA Polyclonal Antibody | anti-NAAA antibody

NAAA, CT (NAAA, ASAHL, PLT, N-acylethanolamine-hydrolyzing acid amidase, Acid ceramidase-like protein, N-acylsphingosine amidohydrolase-like) (Azide free) (HRP)

Cat. Num. AAA6323631

• Gene Names: NAAA; PLT; ASAHL
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: NAAA; Polyclonal Antibody; CT (NAAA; ASAHL; PLT; N-acylethanolamine-hydrolyzing acid amidase; Acid ceramidase-like protein; N-acylsphingosine amidohydrolase-like) (Azide free) (HRP); N-acylsphingosine amidohydrolase-like; anti-NAAA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-NAAA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: NAAA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 330-359 amino acids from the C-terminal region of human NAAA.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NAAA Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the NAAA antibody detected the NAAA protein (arrow).)

Related Product Information for anti-NAAA antibody

This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-NAAA antibody

Antibodies; Enzymes

References

Wang, J., et al. J. Biochem. 144(5):685-690(2008). Muller, T.D., et al. Child Adolesc Psychiatry Ment Health 2 (1), 33 (2008): Schulze, H., et al. Biol. Chem. 388(12):1333-1343(2007). Zhao, L.Y., et al. Biochim. Biophys. Acta 1771(11):1397-1405(2007). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 109148548
• NCBI GeneID: 27163
• NCBI Accession #: NP_001035861.1
• NCBI GenBank Nucleotide #: NM_001042402.1
• UniProt Accession #: Q02083; Q5KTF2; Q96EY2; Q9BRA8
• Molecular Weight: 40,066 Da
• NCBI Official Full Name: N-acylethanolamine-hydrolyzing acid amidase isoform 2
• NCBI Official Synonym Full Names: N-acylethanolamine acid amidase
• NCBI Official Symbol: NAAA
• NCBI Official Synonym Symbols: PLT; ASAHL
• NCBI Protein Information: N-acylethanolamine-hydrolyzing acid amidase; ASAH-like protein; acid ceramidase-like protein
• UniProt Protein Name: N-acylethanolamine-hydrolyzing acid amidase
• Protein Family: N-acylethanolamine-hydrolyzing acid amidase
• UniProt Gene Name: NAAA
• UniProt Synonym Gene Names: ASAHL; PLT; ASAH-like protein
• UniProt Entry Name: NAAA_HUMAN

NCBI Description

This gene encodes an N-acylethanolamine-hydrolyzing enzyme which is highly similar to acid ceramidase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ASAHL: Degrades bioactive fatty acid amides to their corresponding acids, with the following preference: N- palmitoylethanolamine > N-myristoylethanolamine > N- lauroylethanolamine = N-stearoylethanolamine > N- arachidonoylethanolamine > N-oleoylethanolamine. Also exhibits weak hydrolytic activity against the ceramides N- lauroylsphingosine and N-palmitoylsphingosine. Belongs to the acid ceramidase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: lysosome; cytoplasm

Molecular Function: hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds; transcription factor binding

Biological Process: lipid metabolic process