Rabbit NAA50 Polyclonal Antibody | anti-NAA50 antibody

Anti-NAA50 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102803

• Gene Names: NAA50; SAN; MAK3; NAT5; hSAN; NAT13; NAT5P; hNAT5; NAT13P
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: NAA50; Polyclonal Antibody; Anti-NAA50 Antibody; Rabbit Polyclonal; NAA50 Antibody; Rabbit PAb; Antigen Affinity Purified; N(alpha)-acetyltransferase 50; NatE catalytic subunit; Anti-MAK3 Antibody; Anti-NAT13 Antibody; Anti-NAT13P Antibody; Anti-NAT5 Antibody; Anti-NAT5P Antibody; Anti-SAN Antibody; anti-NAA50 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human NAA50
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-NAA50 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the Human NAA50
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human NAA50, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-NAA50 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:19 kDa Observed band size:19 kDa)

Related Product Information for anti-NAA50 antibody

Naa50 (also known as San) has previously been shown to play a role in sister-chromatid cohesion in metazoans. The gene separation anxiety (san) encodes Naa50/San, an N-terminal acetyltransferase required for chromosome segregation during mitosis. Naa50/San N-terminally acetylates the nascent Scc1 polypeptide, and that this co-translational modification is subsequently required for the establishment and/or maintenance of sister chromatid cohesion. NAA50, an interactor of EDR1, plays an important role in regulating the tradeoff between plant growth and defense. Plants lacking NAA50 display severe developmental defects as well as induced stress responses. Reduction of NAA50 expression results in arrested stem and root growth as well as senescence. The loss of NAA50 results in constitutive ER stress signaling, indicating that NAA50 may be required for the suppression of ER stress.

References

Rong Z, et al. (2016) Opposing functions of the n-terminal acetyltransferases naa50 and nata in sister-chromatid cohesion. J Biol Chem 291 (36): 19079-19091.Ribeiro AL, et al. (2016) Naa50/san-dependent n-terminal acetylation of scc1 is potentially important for sister chromatid cohesion. Sci Rep 6 39118.Neubauer M, et al. (2020) Loss of the acetyltransferase naa50 induces endoplasmic reticulum stress and immune responses and suppresses growth. Plant Physiol 183 (4): 1838-1854.

NCBI and Uniprot Product Information

• NCBI GI #: 13376735
• NCBI GeneID: 80218
• NCBI Accession #: NP_079422.1
• NCBI GenBank Nucleotide #: NM_025146.2
• UniProt Accession #: Q9GZZ1; Q68DQ1; D3DN74
• Molecular Weight: 9,465 Da
• NCBI Official Full Name: N-alpha-acetyltransferase 50
• NCBI Official Synonym Full Names: N(alpha)-acetyltransferase 50, NatE catalytic subunit
• NCBI Official Symbol: NAA50
• NCBI Official Synonym Symbols: SAN; MAK3; NAT5; hSAN; NAT13; NAT5P; hNAT5; NAT13P
• NCBI Protein Information: N-alpha-acetyltransferase 50; Mak3 homolog; N-acetyltransferase 13 (GCN5-related); N-acetyltransferase 5; N-acetyltransferase san homolog; N-alpha-acetyltransferase 50, NatE catalytic subunit; natE catalytic subunit; separation anxiety
• UniProt Protein Name: N-alpha-acetyltransferase 50
• Protein Family: N-alpha-acetyltransferase
• UniProt Gene Name: NAA50
• UniProt Synonym Gene Names: MAK3; NAT13; NAT5; hNAT5; hSAN
• UniProt Entry Name: NAA50_HUMAN

Uniprot Description

NAA50: Probable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity. Belongs to the acetyltransferase family. GNAT subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Acetyltransferase; EC 2.3.1.-

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasm

Molecular Function: peptide alpha-N-acetyltransferase activity; protein binding; H4 histone acetyltransferase activity

Biological Process: N-terminal protein amino acid acetylation