Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat N-Acetylgalactosaminidase Alpha (NAGa) Polyclonal Antibody | anti-NAGa antibody
Polyclonal Antibody to N-Acetylgalactosaminidase Alpha (NAGa)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
N-Acetylgalactosaminidase Alpha (NAGa); Polyclonal Antibody; Polyclonal Antibody to N-Acetylgalactosaminidase Alpha (NAGa); anti-NAGa antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against NAGa. It has been selected for its ability to recognize NAGa in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- IQD SWKSVLSILD WFVKHQDILQ PVSGPGHWND PDMLLIGNFG LSFDESRAQM ALWTVLAAPL FMSTDLRTIS PQNIDILQNP LLIKINQDPL GIQGRLIFKS KSHIEVFKRN LSDDASALVF FSRRTDMPYH FHCSLLELNY PKGSVYEGQN VFTGDIISGL HPETNFTVII NPSGVVMWYL YPVKGLGIYT MMSQL
MGHHHHHHSGSEF- IQD SWKSVLSILD WFVKHQDILQ PVSGPGHWND PDMLLIGNFG LSFDESRAQM ALWTVLAAPL FMSTDLRTIS PQNIDILQNP LLIKINQDPL GIQGRLIFKS KSHIEVFKRN LSDDASALVF FSRRTDMPYH FHCSLLELNY PKGSVYEGQN VFTGDIISGL HPETNFTVII NPSGVVMWYL YPVKGLGIYT MMSQL
Sequence Length
411
Applicable Applications for anti-NAGa antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant NAGa (Ile218~Leu415) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2080008
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
46,871 Da
NCBI Official Full Name
alpha-N-acetylgalactosaminidase
NCBI Official Synonym Full Names
alpha-N-acetylgalactosaminidase
NCBI Official Symbol
Naga
NCBI Protein Information
alpha-N-acetylgalactosaminidase
UniProt Protein Name
Alpha-N-acetylgalactosaminidase
Protein Family
UniProt Gene Name
Naga
Uniprot Description
Removes terminal alpha-N-acetylgalactosamine residues from glycolipids and glycopeptides. Required for the breakdown of glycolipids ().
Similar Products
Product Notes
The NAGa naga (Catalog #AAA2003760) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to N-Acetylgalactosaminidase Alpha (NAGa) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's N-Acetylgalactosaminidase Alpha (NAGa) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NAGa naga for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- IQD SWKSVLSILD WFVKHQDILQ PVSGPGHWND PDMLLIGNFG LSFDESRAQM ALWTVLAAPL FMSTDLRTIS PQNIDILQNP LLIKINQDPL GIQGRLIFKS KSHIEVFKRN LSDDASALVF FSRRTDMPYH FHCSLLELNY PKGSVYEGQN VFTGDIISGL HPETNFTVII NPSGVVMWYL YPVKGLGIYT MMSQL. It is sometimes possible for the material contained within the vial of "N-Acetylgalactosaminidase Alpha (NAGa), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
